Sample Preparation & Gel Electrophoresis Troubleshooting

Electric currents, wires, leads, combs, leaks… so many opportunities for trouble. But we still use gels, because electrophoresis remains an effective way to separate proteins — so that the results of antibody-based immunodetection can be fairly unequivocal.

Click on the Sample Preparation & Gel Electrophoresis topics to read about the possible causes and remedies:

No Bands or Gel Front

Sample Doesn't Sink to the Bottom of the Well

Sample Leaking Out of Well

Bands are Smeared Vertically

Too Many Bands

Gel Running Unusually Slowly

Gel Running Unusually Fast

Protein Bands Too Close Together (Not Completely Resolved)

Leftmost and/or Rightmost Bands of the Gel are Distorted

Bands Form Smiling Shapes

Materials
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