The Duolink® PLA technology for protein detection offers a simple and straightforward protocol that has a set of defined steps. This section will delineate important reminders that will ensure your Duolink® PLA experiment proceeds smoothly.
Despite all precautions, and the use of some excellent tips and trick, sometimes the results are not as expected. Lack of signal in a positive control or high background are two of the most common problems that are often seen when performing immunodetection experiments, and may also occur with the Duolink® PLA technology. The tables below list the probable causes and suggested solutions to get around these common problems. If problems remain, contact our Technical Service online or at (800) 325-5832.
This section provides answers to some frequently asked questions (FAQs). However, some information is proprietary and cannot be disclosed. This includes questions pertaining to specific composition of reagents and buffers, oligonucleotide length and sequences, or the conjugation chemistries of the Duolink® PLA Probes and Duolink® Probemaker kits.
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