MilliporeSigma
HomeProtein PurificationAmicon® Pro Purification System – Protein Purification

Amicon® Pro Purification System – Protein Purification

The Amicon® Pro Purification System is an all-in-one centrifugal device for the protein purification workflow. Traditional protein purification is a long process with many steps and multiple tubes, often resulting in protein degradation and loss. Avoid the risks associated with sample transfer and reduce hands-on time when you bind, wash, elute and/or concentrate your protein in the all-in-one Amicon® Pro purification system. Regardless of your workflow, this virtually touchless device delivers consistent, accurate sample preparation, resulting in reliable recovery, uncompromised purity and easy data generation.

The Amicon® Pro Purification System Workflow

Bind, wash, elute and/or concentrate your protein in this all-in-one centrifugal device.

The Amicon® Pro Purification System Workflow

Elute, Don't Dilute

Excellent Recovery, Regardless of Scale

elute dont elute

Figure 1.While three elution steps were required for the smaller spin columns (left), a single spin in the Amicon® Pro device was sufficient to recover > 90% of resin-bound protein in an average of 45 µL final volume*.

Excellent recovery

Figure 2.Protein recovered increases linearly with respect to packed resin volume, offering flexibility in experimental scale. Whether you are using a few microliters or a milliliter of resin, Amicon® Pro purification yields consistent recovery.

Compare the Amicon® Pro Workflow with Traditional Methods

a Process time excludes 60 minute incubation for both protocols.
b Gravity column process includes column setup, purification and assessment of eluted fractions.

Materials
Loading
Sign In To Continue

To continue reading please sign in or create an account.

Don't Have An Account?