Accurately and consistently measuring the total protein concentration in a sample can generate more reliable immunoassays, more informative Western blots of lysates, and streamlined optimization of protein purification. Infrared spectroscopy is a long-standing, accepted method for accurate protein quantitation; however, traditionally, this technology required significant spectroscopy expertise and large instruments.
The Direct Detect system has made infrared spectroscopy accessible to any protein laboratory, benefiting researchers performing several protein quantitation procedures a week, and especially researchers who are dissatisfied with other quantitation methods, (such as UV-Vis spectroscopy, and Bradford, BCA, and Lowry assays).
After generating a single-time standard curve that gets stored in the instrument, just spot 2 μL of sample and triplicate and blank onto an assay-free membrane card and inserting it into the spectrometer. The system reports the average protein concentration of 3 spots and displays the IR spectrum of the sample.
Video. Direct Detect Infrared Spectrometer helps one customer quantitate total protein
while saving sample
Figure 1.Measuring protein concentration without interference from SDS, thanks to nonoverlapping IR signals in the Direct Detect spectrometer.
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