Dot blotting is an ideal technique for quickly assessing the levels of a target antigen across many samples at once. Also, it is a popular method for epitope mapping and screening antibodies for target specificity.

Dot/Slot (Filtration) blotting topics: possible causes and remedies:

Detection of transferrin on a dot blot of human serum using Immobilon® Western Chemiluminescent HRP Substrate.

Figure 1.Detection of transferrin on a dot blot of human serum using Immobilon® Western Chemiluminescent HRP Substrate.

Slow or No Filtration of the Sample Through the Membrane

Little or No Protein Observed on the Blot

Stained Blot Is Not Uniform

Protein Smeared Across the Top of the Membrane

Protein Smeared Across the Back of the Membrane

Materials
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