Western Blot Analysis of Immunoprecipitation (IP-Western)

IP-Western analysis remains a popular technique for identifying protein-protein interactions and identifying unknown proteins in a multi-protein complex. The steps include cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.

Click on the Western Blot Analysis of Immunoprecipitation (IP-Western) topics to read about the possible causes and remedies:

High Background

No Target Protein Eluted, or Not Enough Target Protein Eluted

Extra Band(s) at 50 kDa and 20 kDa on Final Western Blot that May Mask Band of Interest

Guide to Choosing Immunoprecipitation Beads

Guide to Choosing Immunoprecipitation Beads
Materials
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