Western Blot Analysis of Immunoprecipitation (IP-Western)

IP-Western analysis remains a popular technique for identifying protein-protein interactions and identifying unknown proteins in a multi-protein complex. The steps include cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.

Click on the Western Blot Analysis of Immunoprecipitation (IP-Western) topics to read about the possible causes and remedies:

High Background

No Target Protein Eluted, or Not Enough Target Protein Eluted

Extra Band(s) at 50 kDa and 20 kDa on Final Western Blot that May Mask Band of Interest

Guide to Choosing Immunoprecipitation Beads

Guide to Choosing Immunoprecipitation Beads

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.