Most Western blot users visualize transferred proteins using stains, such as Ponceau S or Coomassie® Blue. However, users of PVDF membrane have the opportunity to assess transfer efficiency using transillumination. Areas of PVDF coated with transferred protein are capable of wetting out in 20% methanol while the surrounding areas of PVDF are not. In the areas where the PVDF wets, it becomes optically transparent, allowing visualization of protein bands using backlighting and photographic archiving.

Click on the protein visualization topics to read about the possible causes and remedies:

Poor Detection by Transillumination

Weak or Uneven Stain

Uneven/Splotchy Results

High Background Staining