436143

Dodecilsulfatosódico

ACS reagent, ≥99.0%

• Sinónimos: Dodecil sulfato de sodio, Dodecil sulfato sodium salt, Lauril sulfato sodium salt, Laurilsulfato de sodio, SDS
• Fórmula lineal: CH₃(CH₂)₁₁OSO₃Na
• CAS Number: 151-21-3
• Peso molecular: 288.38
• Beilstein: 3599286
• Número CE: 205-788-1
• Número MDL: MFCD00036175
• Código UNSPSC: 12161900
• eCl@ss: 39093306
• ID de la sustancia en PubChem: 24867284
• NACRES: NA.21

Propiedades

• grado: ACS reagent
• Nivel de calidad: 200
• descripción: anionic; 62
• Ensayo: ≥99.0%
• Formulario: solid
• mol peso: 288.38 g/mol
• impurezas: ≤0.06 meq/g Titr. Base; ≥96.0% fatty alcohols; <4.0% unsulfated alcohols
• pérdida: ≤1.0% loss on drying
• mp: 204-207 °C (lit.)
• solubilidad: H₂O: soluble 1 gm in 10 ml (opalescent solution)
• densidad: 1.03 g/cm³ at 20 °C
• trazas de catión: heavy metals (as Pb): ≤0.002%
• λ: 3 %H₂O reference
• Absorción UV: λ: 220-350 nm A_max: ≤0.1
• HLB: 40
• cadena SMILES: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
• InChI: 1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
• Clave InChI: DBMJMQXJHONAFJ-UHFFFAOYSA-M

Descripción

Descripción general

Sodium dodecyl sulfate (SDS), also known as sodium lauryl sulfate, is a versatile anionic surfactant with a broad range of applications in cell biology, biochemistry, and various industries. Its amphiphilic nature, characterized by a hydrophobic hydrocarbon tail and a hydrophilic sulfate head group, makes it an effective detergent capable of solubilizing and denaturing proteins, lipids, and other hydrophobic molecules.

In protein biochemistry, SDS plays a pivotal role in protein extraction and SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Its ability to denature proteins by disrupting their secondary and tertiary structures allows for their uniform migration through a polyacrylamide gel matrix based on their molecular weight. This technique, SDS-PAGE, has become an indispensable tool for analyzing protein size and purity.

Beyond protein analysis, SDS also finds applications in nucleic acid extraction and hybridization techniques. Its detergent properties aid in the lysis of cells and the solubilization of cellular components, facilitating the extraction of DNA and RNA. This makes SDS a valuable reagent in various molecular biology protocols, including PCR (polymerase chain reaction) and Southern blotting. Sodium dodecyl sulfate stands as a versatile and widely used surfactant with a diverse range of applications due to its ability to solubilize, denature, and separate biological molecules for cell biology and biochemical research.

Aplicación

Detergente aniónico.

Sodium dodecyl sulfate was used as a test compound for studying that whether Bacillus amyloliquefaciens KPS46 can induce priming, signaling, protein production as well as systemic protection in soybean (Glycine max L.) against several diseases.[1]

Acciones bioquímicas o fisiológicas

Sodium dodecyl sulfate (SDS) is an anionic detergent that can form complexes with protein through hydrophobic interactions. Studies have reported that the hydrodynamic functions of protein-SDS complexes are governed by the length of their polypeptide chains. Thus, SDS-based electrophoretic techniques can separate protein molecules based on their molecular weights.[2] Additionally, SDS can solubilize cell membranes and can extract membrane-bound proteins.[3]

Características y beneficios

• Highly versatile surfactant for your molecular biology and biochemical research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Otras notas

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Información de seguridad

• Pictogramas: GHS02,GHS05,GHS07
• Palabra de señalización: Danger
• Frases de peligro: H228,H302 + H332,H315,H318,H335,H412
• Consejos de prudencia: P210 - P273 - P280 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Clasificaciones de peligro: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 4.1B - Flammable solid hazardous materials
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): 338.0 °F
• Punto de inflamabilidad (°C): 170 °C
• Equipo de protección personal: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges