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A0701

Agarosa, baja temperatura de gelificación

Type VII-A

Sinónimos:

2-hidroxietil agarosa

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A ustedes/SKUDisponibilidadPrecio
25 g

Disponible para enviar HOYdesdeMILWAUKEE

$374.00
100 g

Disponible para enviar HOYdesdeMILWAUKEE

$1,180.00
$1,003.00

Acerca de este artículo

Número CAS:
UNSPSC Code:
41105317
NACRES:
NA.21
MDL number:

$374.00


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type

Type VII-A

Quality Segment

form

powder

technique(s)

electrophoresis: suitable

impurities

≤7% water

ash

≤0.4%

turbidity

≤4 NTU (1.5% gel)

EEO

≤0.12

mp

65 °C±1 °C

transition temp

gel point 26 °C ±2 °C (1.5% gel)

gel strength

≥250 g/cm2 (1% gel)

anion traces

sulfate (SO42-): ≤0.4%

SMILES string

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

General description

Agarose is a polymer extracted from agar or agar-bearing marine algae. This purified linear galactan hydrocolloid comprises alternating co-polymers D-galactose and 3,6-anhydro-L-galactopyranose units connected by α-(1→3) and β-(1→4) glycosidic bonds. Agarose is highly biocompatible due to its variable mechanical and diffusion properties.[1]

Application

Excellent for in-gel enzymatic reactions and cloning assays and for recovery of heat-labile samples after electrophoresis
Agarose has been used:
  • to encapsulate Escherichia coli on a hydrogel in tissue culture[1]
  • to entrap Aliivibrio fischeri on a disposable card involved in designing of toxicity biosensors
  • as a the dispersed phase of emulsion during preparation agar beads

Biochem/physiol Actions

Agarose can be used as a gelling agent, to separate nucleic acids electrophoretically because its gels have larger pore sizes than polyacrylamide gels at low concentrations. Unlike polyacrylamide, the consistency of the gels is more solid (but also less elastic) It is also employed to determine cross reaction in immunoelectrophoresis (IEP) and Ouchterlony (double diffusion) plates in which antibody-antigen precipitin lines are studied. Agarose is used to make gel plates or overlays for cells in tissue culture. In addition, it is also used to form a gel matrix (either beaded and/or crosslinked) which can be used in chromatographic separations.

Analysis Note

A continuación se presenta una lista de las propiedades asociadas a nuestras agarosas:
Contenido de sulfatos - utilizados como un indicador de pureza, ya que los sulfatos constituyen el principal grupo iónico presente.
Fuerza del gel - la fuerza que debe aplicarse a un gel para provocar su fractura.
Punto de gelificación - la temperatura a la cual una disolución acuosa de agarosa forma un gel cuando se enfría. Las disoluciones de agarosa exhiben histéresis en la transición de líquido a gel - es decir, su punto de gelificación no es el mismo que su temperatura de fusión.
Electroendosmosis (EEO) - movimiento de líquido a través del gel. En un gel de agarosa, los grupos aniónicos están pegados a la matriz y no pueden moverse, pero los contra-cationes disociables pueden migrar en la matriz hacia el cátodo, dando lugar a EEO. Dado que el movimiento electroforético de los biopolímeros suele ser en dirección al ánodo, la EEO puede alterar las separaciones debido a la convección interna.

Other Notes

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Este artículo
A4018A2576A0169
EEO

≤0.12

EEO

≤0.10

EEO

≤0.12

EEO

0.09-0.13

gel strength

≥250 g/cm2 (1% gel)

gel strength

≥200 g/cm2 (1% gel)

gel strength

≥300 g/cm2 (1.5% gel)

gel strength

≥1200 g/cm2 (1% gel)

form

powder

form

powder

form

powder

form

powder

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

electrophoresis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

anion traces

sulfate (SO42-): ≤0.4%

anion traces

-

anion traces

sulfate (SO42-): ≤0.14%

anion traces

sulfate (SO42-): ≤0.20%


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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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