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MilliporeSigma

71736

Sigma-Aldrich

Sodium dodecyl sulfate solution

BioUltra, Molecular Biology, 10% in H2O

Sinónimos:

Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate solution

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About This Item

Fórmula lineal:
CH3(CH2)11OSO3Na
CAS Number:
Peso molecular:
288.38
Beilstein:
3599286
Número MDL:
Código UNSPSC:
12161902
eCl@ss:
39093306
ID de la sustancia en PubChem:
NACRES:
NA.26

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grado

Molecular Biology

descripción

anionic

Línea del producto

BioUltra

Formulario

liquid

mol peso

288.38 g/mol

concentración

10% in H2O

técnicas

HPLC: suitable
protein quantification: suitable

impurezas

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

5.0-6.5

densidad

1.01 g/mL at 20 °C

trazas de anión

chloride (Cl-): ≤50 mg/kg
phosphate (PO43-): ≤1 mg/kg

trazas de catión

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

Absorción UV

λ: 260 nm Amax: 0.3
λ: 280 nm Amax: 0.2

aplicaciones

(Western Blot)

cadena SMILES

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

Clave InChI

DBMJMQXJHONAFJ-UHFFFAOYSA-M

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Este artículo
42801871725L6026
form

liquid

form

liquid

form

powder

form

powder

grade

for molecular biology

grade

-

grade

for molecular biology

grade

-

technique(s)

HPLC: suitable, protein quantification: suitable

technique(s)

-

technique(s)

-

technique(s)

-

density

1.01 g/mL at 20 °C

density

-

density

0.455 g/cm3

density

0.496 g/cm3

product line

BioUltra

product line

-

product line

BioUltra

product line

BioXtra

mol wt

288.38 g/mol

mol wt

-

mol wt

micellar avg mol wt 18,000

mol wt

micellar avg mol wt 18,000

Aplicación

Sodium dodecyl sulfate solution has been used:
  • in the preparation of 2x extraction buffer[1]
  • as a component in elution buffer for immunoprecipitation[2]
  • in SDS–polyacrylamide gel electrophoresis (PAGE) analysis[3][4]

Acciones bioquímicas o fisiológicas

Sodium dodecyl sulfate (SDS)/lauryl sulfate, is an ionic detergent has the ability to disrupt biological membranes. It can block ribonuclease (RNase) and deoxyribonuclease (DNase) activity. SDS is one of the main constituents of several reagents, that are used to purify nucleic acids.[5] It is also considered as a permeability enhancer in oral formulations.[6]

Otras notas

In large-scale phenol extraction of RNA - promotes the dissociation of protein from nucleic acids when extracting from biological material[7]

Pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Skin Irrit. 2

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Certificados de análisis (COA)

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Questions

1–3 of 3 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. I WOULD LIKE TO REQUEST THE QUALITY CONFIRMATION OF %CONCENTRATION, SINCE CONCENTRATION IS 10% IN WATER, THERE'S NO INFORMATION THAT WHAT'S % TYPE [%W/V OR %V/V] REF.PRODUCT CODE: 71736

    1 answer
    1. This product is 10% (w/w) solution.

      Helpful?

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