Low pressure liquid chromatography

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facet applications:Low pressure liquid chromatography

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Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Maintenance of Multimodal Chromatography Media and Storage Conditions

This page describes the maintenance of media and storage conditions for multimodal chromatography using Cytiva products.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

UHPLC Analysis of Paraquat and Diquat on Supel™ Carbon LC

Ultra-high performance liquid chromatographic (UHPLC) separation of polar herbicides, paraquat and diquat using SupelTM Carbon LC column with baseline resolution, excellent peak shape, and good lot-to-lot reproducibility.

Characteristics of Dextrin Sepharose® High Performance Products

This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.

Capto Adhere Anion Exchanger for MAbs & Bind/Elute

Capto adhere from Cytiva is a multimodal strong anion exchanger for BioProcess applications.

Capto MMC

Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.

Performing a Purity and Homogeneity Check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Immobilized Metal Chelate Affinity Chromatography (IMAC)

How to separate proteins and peptides with afﬁnity for metal ions by immobilized metal chelate afﬁnity chromatography.

QuEChERS Cleanup and Analysis of Veterinary Drugs in Milk by LC/MS/MS

Use QuEChERS methods to analyze samples of milk for veterinary drugs.

Ascentis^® Express Peptide ES-C18 U/HPLC Columns

Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results

Ephedrine/Pseudoephedrine Determination

Discovery® C18 column aids in HPLC method development for quantifying ephedrine and pseudoephedrine in traditional Chinese medicine.

UHPLC Vitamin D Analysis on Supel™ Carbon LC Columns

UHPLC separation of Vitamin D Metabolites using Supel™ Carbon LC column ensures excellent peak shape and sensitivity.

Determination of Chlorogenic acid and Baicalin in compounded Lonucerae Japonicae Flos with Purospher® STAR RP-18e

Extraction and analysis of chlorogenic acid and baicalin active ingredients in traditional Chinese medicine (TCM) according to Chinese pharmacopeia monograph by HPLC with Purospher® RP-18e column

Protein G and Protein A Bind to Different IgG

This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.

LRA (Lipid Removal Agent) for Biopharmaceutical Production

Lipids are naturally-occurring hydrocarbons that are poorly soluble in water and soluble in non-polar solvents such as ether and chloroform.

Viral Clearance Using Capto™ Adhere

Description of viral clearance using Capto adhere from Cytiva.

Bind/Elute vs Flowthrough Mode in Multimodal Chromatography

This page describes the difference between bind/elute and ﬂowthrough modes in multimodal chromatography using Cytiva products.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Purification or Removal of Proteins and Peptides

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.

Purification or Removal of Viruses including Adeno-associated Virus

This page shows how to purify or remove viruses with a Capto DeVirS, AVB Sepharose High Performance from Cytiva.

Purification using GST SpinTrap™

This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Polishing of MAbs Using Capto Adhere ImpRes in Bind/Elute Mode

In these studies, the binding capacity for MAbs and the efficiency in the clearance of impurities using Capto adhere ImpRes in bind/elute mode was evaluated.

Plasmid purification by ion exchange chromatography with Genopure kits

Ion exchange chromatography is employed by the Genopure Plasmid Midi Kit and the Genopure Plasmid Maxi Kit.

Setting Pressure Limits for Size Exclusion Chromatography

Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.

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