[Saltar al contenido](https://www.sigmaaldrich.com#main-content) [](https://www.sigmaaldrich.com/US/es) Productos Carrito0 USES Productos ProductosAplicacionesServiciosRecursosApoyo [Inicio de sesión / Registrarse](https://www.sigmaaldrich.com/oidc-sign-in) [Búsqueda de pedido](https://www.sigmaaldrich.com/US/es/order-lookup) [Pedido rápido](https://www.sigmaaldrich.com/US/es/quick-order) Carrito0 [Home](https://www.sigmaaldrich.com/US/en)[Solid Phase Microextraction (SPME)](https://www.sigmaaldrich.com/US/en/applications/analytical-chemistry/sample-preparation/solid-phase-microextraction)Manual SPME Sampling # Manual SPME Sampling [Learn More About SPME](https://www.sigmaaldrich.com/US/en/applications/analytical-chemistry/sample-preparation/solid-phase-microextraction) Beginners should consider using a manual SPME fiber and holder. The manual system allows the analyst to optimize the extraction process by evaluating different fibers and extraction conditions in a consistent manner. Begin the extraction process by placing the sample into the vial puck on the hotplate. The vial puck allows users to easily index the vial to begin sample agitation. Insert the holder into the sampling stand, passing the needle through the septum that seals the sample vial. Depress the plunger down to expose the fiber to the sample liquid or headspace above the sample. Start the timing of exposure of the SPME fiber for reproducible extractions. After a predetermined time, the SPME fiber is retracted into the protective needle and transferred to the GC for analysis. Inject the SPME fiber into the GC injection port, where the collected organics are quickly transferred to the GC column by heating the fiber. After the thermal transfer of analytes is completed, the fiber is removed and inserted in the next sample vial for extraction. __Related Articles__ - [Biodiesel](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/gas-chromatography/biodiesel) - [Automated Solid Phase Microextraction (SPME) Sampling](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/automated-spme-sampling) - [Bioanalysis with SPME](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/bioanalysis-with-spme) - [BioSPME for Plasma Protein Binding Assay: Details of Method Development and Optimization](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/biospme-plasma-protein-binding-assay) - [Solid Phase Microextraction: Frequently Asked Questions](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/faq) - [GC/MS/MS Analysis of BPA in Energy Drink](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/gc-ms-ms-analysis-of-bisphenol-a-bpa-in-a-fruit-flavored-energy-drink-cla010218a) - [Rugged SPME Sampling](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/nitinol-core-spme-fibers) - [SPME Fiber Performance Tips for Volatiles & Semi-Volatiles](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/performance-tips) - [View More](https://www.sigmaaldrich.com/US/en/search/facet-search?focus=sitecontent&term=facet-search) Arriba __Inicie sesión para continuar.__ Para seguir leyendo, inicie sesión o cree una cuenta. Iniciar sesión__¿No tiene una cuenta?__Registrar Soporte[Atención al cliente](https://www.sigmaaldrich.com/US/es/support/customer-support)[Contáctenos](https://www.sigmaaldrich.com/US/es/collections/offices)[Preguntas frecuentes](https://maestro.my.site.com/knowledgeportal/s/)[Fichas de datos de seguridad (FDS)](https://www.sigmaaldrich.com/US/es/documents-search?tab=sds)[Certificados (COA/COO)](https://www.sigmaaldrich.com/US/es/documents-search?tab=coa)[Calidad y normativa](https://www.sigmaaldrich.com/US/es/life-science/quality-and-regulatory-management)[Calculadoras y aplicaciones](https://www.sigmaaldrich.com/US/es/support/calculators-and-apps)[Seminarios web](https://www.sigmaaldrich.com/US/es/collections/webinars) Pedidos[Búsqueda de pedidos](https://www.sigmaaldrich.com/US/es/order-lookup)[Pedido rápido](https://www.sigmaaldrich.com/US/es/quick-order)[Productos personalizados](https://www.sigmaaldrich.com/US/es/services/custom-products)[Soluciones de comercio electrónico](https://www.sigmaaldrich.com/US/es/life-science/ecommerce/ecommerce-solutions) Empresa[Acerca de nosotros](https://www.sigmaaldrich.com/US/es/life-science/about-us)[Responsabilidad](https://www.sigmaaldrich.com/US/es/life-science/ssbi)[Eventos](https://www.sigmaaldrich.com/US/es/collections/events)[Comunicados de prensa](https://www.sigmaaldrich.com/US/es/collections/press)[Programas](https://www.sigmaaldrich.com/US/es/life-science/partnership-programs)[Empleos](https://careers.merckgroup.com/global/en)[Oficinas](https://www.sigmaaldrich.com/US/es/collections/offices) Redes sociales [](https://www.linkedin.com/company/milliporesigma) [](https://x.com/MilliporeSigma) [](https://www.facebook.com/MilliporeSigma) [](https://www.instagram.com/milliporesigma/) [](https://www.youtube.com/@milliporesigma2407/featured) MilliporeSigma __Investigación. Desarrollo. Producción.__ Somos un proveedor líder en la industria global de las ciencias de la vida, con soluciones y servicios para la investigación, el desarrollo y la producción biotecnológicos, y el desarrollo y la producción de terapias farmacológicas. [](https://www.sigmaaldrich.com/US/es) [SolucionesSigma-Aldrich®](https://www.sigmaaldrich.com/US/es/life-science/sigma-aldrich)[SolucionesBio Reliance®](https://www.sigmaaldrich.com/US/es/life-science/bioreliance)[Soluciones Millipore®](https://www.sigmaaldrich.com/US/es/life-science/millipore)[SolucionesSAFC®](https://www.sigmaaldrich.com/US/es/life-science/safc)[SolucionesMilli-Q®](https://www.sigmaaldrich.com/US/es/life-science/milliq)[Soluciones Supelco®](https://www.sigmaaldrich.com/US/es/life-science/supelco) © 2026 Merck KGaA, Darmstadt, Alemania y/o sus filiales. Todos los derechos reservados, incluyendo la minería de textos y datos para el entrenamiento de IA y tecnologías similares. Queda terminantemente prohibida la reproducción de cualquier material del sitio sin permiso. [Condiciones de uso del sitio web](https://www.sigmaaldrich.com/US/es/life-science/legal/site-use-terms)|[Política de privacidad](https://www.sigmaaldrich.com/US/es/life-science/legal/privacy-statement)|[Condiciones generales de venta](https://www.sigmaaldrich.com/US/es/life-science/legal/terms-and-conditions)|[Consentimiento de derechos de autor](https://www.sigmaaldrich.com/US/es/life-science/legal/copyright-consent)|[Mapa del sitio](https://www.sigmaaldrich.com/US/es/site-map) An unknown error has occured.