Merck
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MGAMMAG-300E

Millipore

MILLIPLEX® Mouse IgE Single Plex Magnetic Bead Kit - Isotyping Multiplex Assay

Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulin E (IgE) in mouse serum, plasma and cell culture samples.

eCl@ss:
32161000

Quality Level

species reactivity

mouse

manufacturer/tradename

Milliplex®

assay range

accuracy: 82%
sensitivity: 0.16 ng/mL
standard curve range: 0.8-3500 ng/mL
inter-assay cv: <10%
intra-assay cv: <5%

technique(s)

multiplexing: suitable

compatibility

configured for Single Plex

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

Found only in mammals, Immunoglobulin E (IgE) plays an essential role in allergic reactions and is predominantly associated with type 1 hypersensitivity. IgE is the least abundant immunoglobulin isotype in normal or topic mammals, present at only 0.05% of IgG serum concentrations. It can trigger robust immune reactions, with up to 10 times the typical level detected in the serum of atopic mammals, although increased levels occur only in approximately 50% of mammals with allergic disorders. IgE binds with high affinity to FcRI, found primarily on mast cells and basophils, priming the cells to recognize specific antigens. Once primed and with repeated exposure to the same antigen, these cells release molecules such as histamines, prostaglandins, and leukotrienes, resulting in an “allergic reaction”.

The MILLIPLEX® Mouse IgE Single Plex Magnetic Bead Kit is a single plex kit to be used to measure levels of IgE in only 2 μL of mouse urine, serum, plasma or tissue/cell lysate and culture supernatant samples.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Immune Response

Specificity

Cross-reactivity between these antibodies and those against other immunoglobulins is non-detectable or negligible.

Application

  • Analyte: IgE
  • Recommended Sample type: Serum, plasma, tissue/cell lysates and tissue culture supernatant
  • Recommended Sample dilution: 1:200 dilution for plasma or serum. Tissue/ cell culture supernatants are diluted to approximately 1μg/mL
  • Assay Run Time: One day
  • Research Category: Immunology and Inflammation, Isotyping

Packaging

96 Well Plate

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Certificate of Analysis

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Certificate of Quality

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