Merck
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MGAMMAG-300K

Millipore

MILLIPLEX® Mouse Immunoglobulin Isotyping Magnetic Bead Panel - Isotyping Multiplex Assay

Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulins (Ig) in murine serum, plasma and cell culture samples.

Synonym(s):
Luminex® Mouse Isotyping Assay, Millipore Mouse Isotyping Assay, Mouse IgG, IgA, IgM immunoassay, Mouse Isotyoing Multiplex Assay
eCl@ss:
32161000


To order a Milliplex® kit, please search for your analyte of interest.


Design And Price Your Kit

Quality Level

species reactivity

mouse

manufacturer/tradename

Milliplex®

assay range

accuracy: 100%
(IgM)

accuracy: 85%
(IgG3)

accuracy: 98%
(IgA)

accuracy: 98%
(IgG2b)

sensitivity: 0.18 ng/mL
(IgG2b)

sensitivity: 0.32 ng/mL
(IgG3)

sensitivity: 0.67 ng/mL
(IgG2a)

sensitivity: 0.7 ng/mL
(IgG1)

sensitivity: 1.1 ng/mL
(IgA)

sensitivity: 1.73 ng/mL
(IgM)

standard curve range: 1-900 ng/mL
(IgG2b)

standard curve range: 2-1,500 ng/mL
(IgG1)

standard curve range: 2-1,500 ng/mL
(IgG3)

standard curve range: 2-1,500 ng/mL
(IgM)

standard curve range: 3-2,000 ng/mL
(IgG2a)

standard curve range: 5-3,000 ng/mL
(IgA)

inter-assay cv: 14-17%
intra-assay cv: 5-10%

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

Produced by plasma cells and lymphocytes, immunoglobulins (antibodies) are critically involved in immune response, attaching to antigens, and playing a role in their destruction. Immunoglobulins (Ig) can be classified by isotype, classes that differ in function and antigen response due to structure variability. Six major isotypes have been identified in placental mammals: IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA – all found in normal individuals. Immunoglobulin-deficiency disorders, such as autoimmune disease, some GI conditions, and malignancies, are characterized by specific isotype deficiencies or varying concentrations of one or more isotypes. Disease states can range from the absence of one isotype class or subclass to a total deficiency of immunoglobulin classes.

MILLIPLEX® Mouse Isotyping Bead Panel, measuring mouse IgG subclasses (1, 2a, 2b, 3), IgM, and IgA, has been designed to enable you to measure both classes and subclasses accurately – all in one well. In addition, this panel allows you to choose only those immunoglobulins you need for your experiments.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Immune Response

Application

  • Analytes: IgG1, IgG2a, IgG2b, IgG3, IgA, IgM
  • Recommended Sample type: Serum, plasma, or tissue/cell lysate and culture supernatant
  • Recommended Sample dilution: 1:50,000 diluted plasma or serum, or cell culture supernatant diluted to approximately 1μg/mL Ig well.
  • Assay Run Time: One day
  • Research Category: Immunology and Inflammation, Isotyping

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Linkage

Replaces: MGAM-300

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Certificate of Analysis

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Certificate of Quality

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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