Merck
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11074547001

Roche

Insulin-Transferrin-Sodium Selenite Supplement

suitable for cell culture, lyophilized, pkg of 50 mg (for 5 l medium)

Synonym(s):
ITS

biological source

human serum (transferrin)
synthetic (sodium selenite)

Quality Level

recombinant

expressed in human cells

sterility

non-sterile; 0.2 μm filtered

assay

>98% (Transferrin, SDS-PAGE)
>99% (Insulin, HPLC)
>99% (Sodium selenite)

form

lyophilized

packaging

pkg of 50 mg (for 5 l medium)

manufacturer/tradename

Roche

technique(s)

cell culture | mammalian: suitable

solubility

water: miscible

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... INS(280829)
human ... TFRC(7037)

General description

The Insulin-Transferrin-Sodium Selenite Supplement contains the most essential growth-promoting components of serum-free culture media in an optimized ratio.
Source: Insulin- bovine pancreas; transferrin- human serum; Sodium selenite-synthetic
The insulin-transferrin-sodium selenite (ITS) supplement comprises three of the most crucial growth factors for many cell types. Insulin is a component of serum-free media formulations for all primary cells and cell lines. It stimulates cell growth, increases fatty acid and glycogen synthesis in a serum-free medium. Transferrin is an essential growth factor for many cell types. Selenium is very often necessary for optimal cell growth. ITS permits an appreciable reduction in serum requirements for cellular growth. ITS is also an antioxidant that supports the growth of in vivo derived (IVD) embryos. Insulin promotes embryonic growth and metabolism. The iron transport in the embryo is mediated by transferrin. Sodium selenite protects from oxidative damage and inhibits lipid peroxidation. ITS is useful in human chondrocytes monolayer culture.

Application

The Insulin-Transferrin-Sodium Selenite Supplement is used in the cell culture media as a basal growth-factor supplement to which other cell type-specific nutrients and growth factors are added.

Preparation Note

Working concentration: Insulin, 5 μg/ml, transferrin, 5 μg/ml, sodium selenite, 5 ng/ml (3.0 x 10-8 M)

Storage conditions (working solution): -15 to -25 °C.
Prepare appropriate aliquots and avoid repeated freezing and thawing.

Reconstitution

The insulin-transferrin-sodium selenite supplement should be reconstituted in 5 ml or 25 ml sterile double-dist. water (1000 x concentrated stock solution).
Further dilution with culture medium.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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