Merck
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PHR1939

Supelco

Hydroxyproline

Pharmaceutical Secondary Standard; Certified Reference Material

Synonym(s):
trans-4-Hydroxy-L-proline, (2S,4R)-4-Hydroxypyrrolidine-2-carboxylic acid, Hyp
Empirical Formula (Hill Notation):
C5H9NO3
CAS Number:
Molecular Weight:
131.13
Beilstein:
81441
EC Number:
MDL number:
NACRES:
NA.24

grade

certified reference material
pharmaceutical secondary standard

Quality Level

CofA

current certificate can be downloaded

packaging

pkg of 500 mg

mp

273 °C (dec.) (lit.)

application(s)

pharmaceutical

format

neat

storage temp.

2-30°C

SMILES string

O[C@H]1CN[C@@H](C1)C(O)=O

InChI

1S/C5H9NO3/c7-3-1-4(5(8)9)6-2-3/h3-4,6-7H,1-2H2,(H,8,9)/t3-,4+/m1/s1

InChI key

PMMYEEVYMWASQN-DMTCNVIQSA-N

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General description

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.

Pharmaceutical secondary standards for application in quality control provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.

Hydroxyproline, found exclusively in collagen, is formed from the hydroxylation of proline. It is used as a biomarker to measure the collagen content of various biological samples.

Application

This pharmaceutical secondary standard can also be used as follows:

  • Multi-analysis of acetylcarnitine, proline, hydroxyproline, citrulline, and arginine as possible biomarkers by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in human plasma samples
  • Simultaneous determination of proline and hydroxyproline in plasma samples by (LC-MS/MS using a new fluorescent reagent for pre-column derivatization
  • Development of a micellar electrokinetic chromatography (MEKC) method to measure hydroxyproline in various dairy products upon in-capillary derivatization
  • Quantitative analysis of free prolyl dipeptides, proline, and hydroxyproline by flow-gated capillary electrophoresis combined with laser-induced fluorescence detector from unhydrolyzed urine samples[1]

Other Notes

Natural constituent of animal structural proteins such as collagen and elastin.

Analysis Note

These secondary standards offer multi-traceability to the USP, EP and BP primary standards, where they are available.

Footnote

To see an example of a Certificate of Analysis for this material enter LRAC2898 in the Documents slot below. This is an example certificate only and may not be the lot that you receive.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

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