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X100

Sigma-Aldrich

Triton X-100

laboratory grade

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Synonym(s):
t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
Beilstein:
2315025
MDL number:
PubChem Substance ID:
NACRES:
NA.21

biological source

synthetic

Quality Level

grade

laboratory grade

description

non-ionic

form

liquid

mol wt

micellar avg mol wt 80,000
average mol wt 625

aggregation number

100-155

CMC

0.2-0.9 mM (20-25°C)

mp

6  °C (43 °F)

transition temp

cloud point 65 °C
pour point ~7 °C

solubility

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

HLB

13.5

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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1 of 4

This Item
X100PCT9284T8787
vibrant-m

X100

Triton X-100

-
vibrant-m

X100PC

Triton X-100

-
vibrant-m

T9284

Triton X-100

Premium Grade
vibrant-m

T8787

Triton X-100

-
form

liquid

form

liquid

form

liquid

form

liquid

aggregation number

100-155

aggregation number

100-155

aggregation number

100-155

aggregation number

100-155

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

solubility

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

solubility

water: soluble

solubility

water: soluble

solubility

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

transition temp

cloud point 65 °C, pour point ~7 °C

transition temp

cloud point 65 °C, pour point ~7 °C

transition temp

cloud point 65 °C, pour point ~7 °C

transition temp

cloud point 65 °C, pour point ~7 °C

General description

Triton X-100 has hemolytic property and is used for DNA extraction.
Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton-X has been used:
  • In the permeabilization of cells for immunofluorescence staining
  • As a component of lysis buffer in western blot analysis
  • As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Features and Benefits

  • Ideal for Cell Biology and Biochemical research applications
  • Non-ionic nondenaturing detergent
  • Superior wetting agent and emulsifier
  • Highly versatile surfactant

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Tri-Chek is a trademark of Micro Essential Laboratory, Inc.
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

483.8 °F

Flash Point(C)

251 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.

Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.

Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.

Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.

Protocols

This procedure uses peroxidase and o-dianisidine to measure cholesterol oxidase activity at 500 nm. Cholesterol oxidase is an enzyme that acts on the CH-OH group of the donor with oxygen as the acceptor.

This procedure uses peroxidase and o-dianisidine to measure cholesterol oxidase activity at 500 nm. Cholesterol oxidase is an enzyme that acts on the CH-OH group of the donor with oxygen as the acceptor.

This procedure uses peroxidase and o-dianisidine to measure cholesterol oxidase activity at 500 nm. Cholesterol oxidase is an enzyme that acts on the CH-OH group of the donor with oxygen as the acceptor.

This procedure uses peroxidase and o-dianisidine to measure cholesterol oxidase activity at 500 nm. Cholesterol oxidase is an enzyme that acts on the CH-OH group of the donor with oxygen as the acceptor.

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