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57000

Sigma-Aldrich

Indigo carmine

for microscopy (Bact., Hist.), indicator (pH 11.5-14.0)

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Synonym(s):
Acid Blue 74, Indigo-5,5′-disulfonic acid disodium salt, Indigocarmine
Empirical Formula (Hill Notation):
C16H8N2Na2O8S2
CAS Number:
Molecular Weight:
466.35
Colour Index Number:
73015
Beilstein:
4103904
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

for microscopy (Bact., Hist.)
indicator (pH 11.5-14.0)

Quality Level

form

powder

loss

≤5% loss on drying

pH

3.87 (30 °C, 7.14 g/L)

mp

>340 °C

solubility

methanol: 0.01 g/10 mL, blue to very deep blue

density

0.71 g/cm3 at 29 °C

εmax

≥350 at 603-613 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]S(=O)(=O)c1ccc2NC(\C(=O)c2c1)=C3\Nc4ccc(cc4C3=O)S([O-])(=O)=O

InChI

1S/C16H10N2O8S2.2Na/c19-15-9-5-7(27(21,22)23)1-3-11(9)17-13(15)14-16(20)10-6-8(28(24,25)26)2-4-12(10)18-14;;/h1-6,17-18H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2/b14-13+;;

InChI key

KHLVKKOJDHCJMG-QDBORUFSSA-L

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This Item
1311641747073436
Indigo carmine for microscopy (Bact., Hist.), indicator (pH 11.5-14.0)

57000

Indigo carmine

Indigo carmine certified by the Biological Stain Commission, Dye content 85 %

131164

Indigo carmine

Bromocresol Green Sultone Form for microscopy (Bot., Hist., Vit.), indicator (pH 3.8-5.4)

17470

Bromocresol Green Sultone Form

Indigo carmine analytical standard

73436

Indigo carmine

form

powder

form

solid

form

powder

form

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

cleaning products
cosmetics
food and beverages
personal care

solubility

methanol: 0.01 g/10 mL, blue to very deep blue

solubility

-

solubility

methanol: water (1:1): 0.1 g/10 mL, red

solubility

-

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

-

εmax

≥350 at 603-613 nm

εmax

20,000 at 250-256 nm in water at 0.01 g/L, 29500 at 284-290 nm in water at 0.01 g/L

εmax

≥225 at 418-428 nm in methanol

εmax

-

Application

Indigo carmine has been used in agarose plates to study behavioral feeding assays in Drosophila larvae. It has also been used for macroscopic assessment of intestinal adenomatous polyps isolated from mice.

Biochem/physiol Actions

Indigo carmine is a contrast dye which helps in highlighting mucosal changes. It accumulates in crevices, cysts and foveolae and stains the tissue blue. The abnormal tissue remains translucent pink.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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