Merck
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MAK211

Sigma-Aldrich

Lipolysis Colorimetric Assay Kit

Sufficient for 100 Colorimetric tests

EC Number:
NACRES:
NA.84

detection method

colorimetric

storage temp.

−20°C

Related Categories

General description

Lipolysis is the process of hydrolyzing triglycerides to free fatty acids and glycerol.[1] This process involves the action of adipose TG lipase (ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipase.[2] Lipolysis maintains the energy balance during fasting and exercise by providing a substrate for oxidative metabolism. Lipolysis is regulated by nutritional factors and hormones. Problems with the regulation of lipolysis is associated with obesity, diabetes, and metabolic syndromes.[2]

Suitability

Suitable for the measurement of lipolysis in 3T3-L1 and adipocytes.

Principle

The Lipolysis (3T3-L1) Colorimetric Assay Kit contains a synthetic catecholamine (isoproterenol) that activates β-adrenergic receptors. This activates adenylate cyclase, converting ATP to cAMP. cAMP then activates the hydrolysis of triglycerides by hormone-sensitive lipase. Lipolysis is determined by measuring a colorimetric product with absorbance at 570 nm (A570) proportional to the amount of glycerol present.

Kit Components Only

Product No.
Description

  • Lipolysis Assay Buffer

  • Lipolysis Wash Buffer

  • Glycerol Assay Buffer

  • Glycerol Probe, in DMSO

  • Glycerol Enzyme Mix

  • Glycerol Standard, 100 mM

  • Isoproterenol, 10 mM

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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