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MAK322

Sigma-Aldrich

Collagen Assay Kit

sufficient for 100 fluorometric tests

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

Related Categories

General description

Collagen is the key structural protein of connective tissue and the most abundant protein in mammals. It occurs in many different types and forms with Types I -V being the most common. Aside from the crucial role it plays in the body, it has numerous medical applications such as its use in reconstructive surgery including bone and skin grafts. It is also commonly used in cosmetics due to its anti-aging and skin healing properties.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for biological samples (e.g. plasma, serum, cells) and cosmetic samples (e.g. collagen solution)

Principle

Assay methods available for quantifying collagen currently range from needing extensive hydrolysis procedures with acids and bases to using expensive antibodies and complicated protocols. The collagen assay kit delivers a very simple, safe (non-radioactive), and sensitive method to quantify collagen in samples. In the first step of this procedure, collagen in the sample is enzymatically digested into peptides. Subsequently, the N-terminal glycine containing peptides react with the dye reagent to form a fluorescent complex. The fluorescence intensity of this product, measured at λex/em = 375/465 nm, is directly proportional to collagen concentration in the sample.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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