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MAK329

Sigma-Aldrich

L-Lactate Assay Kit

sufficient for 100 colorimetric tests

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

−20°C

Related Categories

General description

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for lactate determination in serum, plasma, and cell media samples.

Principle

The Lactate Assay Kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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