MAK443

L-Lactate Assay Kit

Sufficient for 100 fluorometric tests

Properties

• Quality Level: 100
• detection method: fluorometric
• relevant disease(s): diabetes; pulmonary disorders; endocrinological disorders; cardiovascular diseases; cancer
• storage temp.: −20°C

Description

General description

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.

Features and Benefits

• Detection limit of 1 μM and linearity up to 50 μM L-lactate in 96-well plate assay.
• Convenient room temperature assay. The procedure involves adding a single working reagent, and reading the fluorescence after 60 minutes.
• Compatible with high-throughput handling systems.

Suitability

The kit is suitable for the quantitative determination of L-lactate (L-lactic acid) and the evaluation of drug effects on its metabolism in serum, plasma, urine, cell culture media, and other biological samples.

Principle

The L-Lactate Assay Kit is based on the lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm, is proportional to the lactate concentration in the sample.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2