Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human holo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Human holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.
holo-Transferrin human has been supplemented in Roswell Park Memorial Institute (RPMI) for proerythroblasts proliferation assay and also in culture media for inductively coupled plasma emission spectroscopy.
50, 100, 500 mg in poly bottle
1 g in poly bottle
Provides iron to cells in a physiologically stable and safe form.
Heat treated at 60 °C (minimum) for a minimum of 10 hours.
Iron content by ICP reported on a lot-specific basis.
Purity by agarose gel electrophoresis.