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Low pressure liquid chromatography

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Non-volatile and Volatile Buffer Systems
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
Selection of Purification Equipment
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
Desalting and Buffer Exchange for Affinity Chromatography of Antibodies
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Troubleshooting Guide for Affinity Chromatography of Tagged Proteins
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
Purification or Removal of Viruses including Adeno-associated Virus
This page shows how to purify or remove viruses with a Capto DeVirS, AVB Sepharose High Performance from Cytiva.
Polishing of MAbs Using Capto Adhere ImpRes in Bind/Elute Mode
In these studies, the binding capacity for MAbs and the efficiency in the clearance of impurities using Capto adhere ImpRes in bind/elute mode was evaluated.
Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
Purification or Removal of Proteins and Peptides
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.
Characteristics of Dextrin Sepharose® High Performance Products
This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.
Maintenance of Multimodal Chromatography Media and Storage Conditions
This page describes the maintenance of media and storage conditions for multimodal chromatography using Cytiva products.
Performing a Purity and Homogeneity Check
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
Affinity Chromatography Troubleshooting
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Capto Adhere Anion Exchanger for MAbs & Bind/Elute
Capto adhere from Cytiva is a multimodal strong anion exchanger for BioProcess applications.
Protein G and Protein A Bind to Different IgG
This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.
Purification Using Protein A-based Chromatography Media
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
Immobilized Metal Chelate Affinity Chromatography (IMAC)
How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.
Immobilized Metal Chelate Affinity Chromatography (IMAC)
How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.
Plasmid purification by ion exchange chromatography with Genopure kits
Ion exchange chromatography is employed by the Genopure Plasmid Midi Kit and the Genopure Plasmid Maxi Kit.
Plasmid purification by ion exchange chromatography with Genopure kits
Ion exchange chromatography is employed by the Genopure Plasmid Midi Kit and the Genopure Plasmid Maxi Kit.
QuEChERS Cleanup and Analysis of Veterinary Drugs in Milk by LC/MS/MS
Use QuEChERS methods to analyze samples of milk for veterinary drugs.
QuEChERS Cleanup and Analysis of Veterinary Drugs in Milk by LC/MS/MS
Use QuEChERS methods to analyze samples of milk for veterinary drugs.
Ascentis® Express Peptide ES-C18 U/HPLC Columns
Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results
Ascentis® Express Peptide ES-C18 U/HPLC Columns
Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results
Ascentis® Express Peptide ES-C18 U/HPLC Columns
Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results
Ascentis® Express Peptide ES-C18 U/HPLC Columns
Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results
Setting Pressure Limits for Size Exclusion Chromatography
Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.
Setting Pressure Limits for Size Exclusion Chromatography
Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.
Setting Pressure Limits for Size Exclusion Chromatography
Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.
Setting Pressure Limits for Size Exclusion Chromatography
Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.
Protein Affinity Chromatography
Affinity chromatography purifies compounds using selective ligands on porous matrix, ensuring specific recovery.
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