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Iron-chelating and free-radical scavenging activities of microwave-processed green tea in iron overload.

Hemoglobin (2006-06-27)
Somdet Srichairatanakool, Sakaewan Ounjaijean, Chonthida Thephinlap, Udompun Khansuwan, Chada Phisalpong, Suthat Fucharoen
ABSTRACT

Secondary iron overload is found in beta-thalassemia (thal) patients because of increased dietary iron absorption and multiple blood transfusions. Excessive iron catalyzes free-radical generation, leading to oxidative damage and vital organ dysfunction. Non-transferrin-bound iron (NTBI) detected in thalassemic plasma is highly toxic and chelatable. Though used to treat iron overload, desferrioxamine (DFO) and deferiprone (L1) also have adverse effects. Green tea (GT) shows many pharmacological effects, particularly antioxidative and iron-chelating capacities. This study was performed to investigate the ability of GT extracts to reduce plasma NTBI concentration and oxidative stress in vitro. The Fe(3+) was found to bind to GT crude extract and form a complex. Green tea crude extract time- and dose-dependently decreased plasma NTBI concentration and counteracted the increase of oxidative stress in both Fe(2+)-EDTA-treated human plasma and erythrocytes. Green tea is a bifunctional natural product that could be relevant for management of iron overload and oxidative stress.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Iron(III) nitrate nonahydrate, BioReagent, suitable for cell culture
Supelco
Iron standard for AAS, analytical standard, 10.00 g/L Fe in nitric acid, traceable to BAM
Sigma-Aldrich
Iron(III) nitrate nonahydrate, ≥99.95% trace metals basis
Sigma-Aldrich
Iron(III) nitrate nonahydrate, ≥99.999% trace metals basis
Sigma-Aldrich
Iron(III) nitrate nonahydrate, ACS reagent, ≥98%