Merck
  • Home
  • Search Results
  • Enhanced NTPDase and 5'-nucleotidase activities in diabetes mellitus and iron-overload model.

Enhanced NTPDase and 5'-nucleotidase activities in diabetes mellitus and iron-overload model.

Molecular and cellular biochemistry (2006-11-23)
Vinícius Ribas Miron, Liliane Bauermann, André Luis Bittencourt Morsch, Rafael Fernandes Zanin, Maísa Corrêa, Adriane Cismoski da Silva, Cínthia Mazzanti, Vera Maria Morsch, Gilberto Ignácio Lunkes, Maria Rosa Chitolina Schetinger
ABSTRACT

The activity of the enzymes NTPDase and 5'-nucleotidase was studied in both diabetes mellitus and an associated model of iron-overload. Rats were divided in five groups: citrate (CC), saline (S), diabetic (D), iron-overload (IO), and diabetic iron-overload (DIO). Diabetes was induced with alloxan (150 mg/kg), and iron-overload was induced with iron-dextran (10 intramuscular applications of +/-80 mg/kg). The enzymatic activities were evaluated in the platelets. The results demonstrated an increase in the activity of NTPDase with substrates ATP and ADP (60% and 120%, respectively; P<0.001), and 5'-nucleotidase (60%, P<0.001). This increase was more intense in the IO and DIO groups. The results obtained in vitro showed an activation in ATP, ADP, and AMP hydrolysis between 1 microM and 1,000 microM ferric nitrate concentrations, being more pronounced at 100 microM and decreasing at 1,000 microM. We concluded that diabetes mellitus in association with iron-overload increased the hydrolysis of adenine nucleotides in platelets, contributing to the abnormalities found in these pathological conditions.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Iron(III) nitrate nonahydrate, BioReagent, suitable for cell culture
Supelco
Iron standard for AAS, analytical standard, 10.00 g/L Fe in nitric acid, traceable to BAM
Sigma-Aldrich
Iron(III) nitrate nonahydrate, ≥99.95% trace metals basis
Sigma-Aldrich
Iron(III) nitrate nonahydrate, ≥99.999% trace metals basis
Sigma-Aldrich
Iron(III) nitrate nonahydrate, ACS reagent, ≥98%