• Home
  • Search Results
  • Toxicity of two types of silver nanoparticles to aquatic crustaceans Daphnia magna and Thamnocephalus platyurus.

Toxicity of two types of silver nanoparticles to aquatic crustaceans Daphnia magna and Thamnocephalus platyurus.

Environmental science and pollution research international (2012-11-13)
Irina Blinova, Jukka Niskanen, Paula Kajankari, Liina Kanarbik, Aleksandr Käkinen, Heikki Tenhu, Olli-Pekka Penttinen, Anne Kahru
ABSTRACT

Although silver nanoparticles (NPs) are increasingly used in various consumer products and produced in industrial scale, information on harmful effects of nanosilver to environmentally relevant organisms is still scarce. This paper studies the adverse effects of silver NPs to two aquatic crustaceans, Daphnia magna and Thamnocephalus platyurus. For that, silver NPs were synthesized where Ag is covalently attached to poly(vinylpyrrolidone) (PVP). In parallel, the toxicity of collargol (protein-coated nanosilver) and AgNO₃ was analyzed. Both types of silver NPs were highly toxic to both crustaceans: the EC50 values in artificial freshwater were 15-17 ppb for D. magna and 20-27 ppb for T. platyurus. The natural water (five different waters with dissolved organic carbon from 5 to 35 mg C/L were studied) mitigated the toxic effect of studied silver compounds up to 8-fold compared with artificial freshwater. The toxicity of silver NPs in all test media was up to 10-fold lower than that of soluble silver salt, AgNO₃. The pattern of the toxic response of both crustacean species to the silver compounds was almost similar in artificial freshwater and in natural waters. The chronic 21-day toxicity of silver NPs to D. magna in natural water was at the part-per-billion level, and adult mortality was more sensitive toxicity test endpoint than the reproduction (the number of offspring per adult).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Silver nitrate, ACS reagent, ≥99.0%
Sigma-Aldrich
Silver nitrate, ReagentPlus®, ≥99.0% (titration)
Sigma-Aldrich
Silver nitrate, 99.9999% trace metals basis
Sigma-Aldrich
Silver nitrate, meets analytical specification of Ph. Eur., BP, USP, 99.8-100.5%
Sigma-Aldrich
Silver nitrate, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%
Sigma-Aldrich
Silver nitrate solution, 2.5 % (w/v) AgNO3 in H2O
Sigma-Aldrich
Silver nitrate, BioXtra, >99% (titration)
Sigma-Aldrich
Silver nitrate on silica gel, extent of labeling: ~10 wt. % loading, +230 mesh
Sigma-Aldrich
Silver nitrate, puriss. p.a., ≥99.5% (AT)
Sigma-Aldrich
Silver nitrate, BioReagent, suitable for plant cell culture, >99% (titration)
Sigma-Aldrich
Silver nitrate, tested according to Ph. Eur.