In this study, a three-bore polyethersulfone (PES) hollow fiber ultrafiltration (UF) membrane with antibacterial properties was prepared by phase inversion, using PES as the membrane material, N,N-dimethylacetamide (DMAC) as solvent, polyvinylpyrrolidone (PVP) and AgNO3 as additives. The silver particles were detected by X-ray photoelectron spectroscopy. The effect of AgNO3 content on the antibacterial properties and separation performance was studied in detail. The membranes showed good antibacterial activity against Escherichia coli after adding AgNO3 and the antibacterial rate of PES/AgNO3 UF membrane with AgNO3 content of 1 wt% could reach 99.9% after running for 48 hours. Moreover, the bovine serum albumin solution filtration results indicated that the PES/AgNO3 membranes had a certain degree of antifouling performance. Therefore, three-bore PES/AgNO3 membranes have a potential application to reduce both bacterial and organic fouling in water treatment.