HomeMicroelectronics & NanoelectronicsApplications of Graphene Oxide and Reduced Graphene Oxide

Applications of Graphene Oxide and Reduced Graphene Oxide

Avery Luedtke, Ph.D.

MilliporeSigma, Milwaukee, WI


Graphene oxide (GO, product numbers 763705 and 777676) is a unique material that can be viewed as a single monomolecular layer of graphite with various oxygen containing functionalities such as epoxide, carbonyl, carboxyl and hydroxyl groups.1,2,3 Interest in GO increased dramatically after graphene, a single layer of graphite, was first isolated and studied.4 It was initially hoped that GO might be a synthetic precursor to graphene.5 When the electrically insulating GO is reduced, the reduced graphene oxide (GO, product number 777684) formed resembles graphene but contains residual oxygen and other heteroatoms as well as structural defects.6,7 A fabrication procedure for rGO that more closely resembles pristine graphene is an outstanding challenge in the field. Nevertheless, since rGO can be made as a thin film from an aqueous dispersion of GO in water and has moderate conductivity, it is attractive for use in electronic devices.8,9,10,11,12 In addition to being components in electronic devices, GO and rGO have been used in nanocomposite materials,13,14 polymer composite materials,13 energy storage,14 biomedical applications,15,16,17 catalysis,18,19,20 and as a surfactant21 with some overlaps between these fields.

Synthesis and Reduction of GO

Many modern procedures for the synthesis of GO are based on the method first reported by Hummers in which graphite is oxidized by a solution of potassium permanganate in sulfuric acid.22 Reduction of GO using hydrazine has been reported.5 However, hydrazine is highly toxic and can potentially functionalize the GO with nitrogen heteroatoms.23 Because of these issues, alternatives to hydrazine including NaBH4,14 ascorbic acid,24 and HI25,26 among others have been used for the reduction of GO. GO can be reduced as a thin film or in an aqueous solution. Reduction methods have been recently reviewed.6


Several electronic devices have been fabricated using GO as a starting material for at least one of the components. One such device is a graphene based field effect transistor (GFET).27,28 Field effect transistors (FETs) that employ rGO have been used as chemical sensors29,30,31 and biosensors. An diagram of GFET sensors is shown in Figure 1.31 GFETs that use functionalized rGO as the semiconductor have been used as biosensors to detect hormonal catecholamine molecules,32 avidin,33 and DNA.34 In another study, GO that was functionalized with glucose oxidase and deposited on an electrode was used as an electrochemical glucose sensor.35

Visible light transparent electrodes are important for both light emitting diodes (LEDs) and solar cell devices. Since GO can be processed in solution, using rGO as a transparent electrode is a convenient alternative to other transparent electrodes such as ITO for these devices.36,37 In addition to being a transparent electrode, rGO has been used as a hole transport layer in polymer solar cells and LEDs.38,39

Gfet sensors

Figure 1.(a) Typical back-gate GFET on Si/SiO2 substrate used as gas sensor. (b) Typical solution-gate GFET on flexible polyethylene terephthalate (PET) substrate used as chemical and biological sensor in aqueous solution. Reproduced from reference 31 with permission from the Centre National de la Recherche Scientifique (CNRS) and The Royal Society of Chemistry.

Energy Storage

Nanocomposites of rGO have been used for high capacity energy storage in lithium ion batteries. In these studies, electrically insulating metal oxide nanoparticles were adsorbed onto rGO to increase the performance of these materials in batteries.40,41,42,43,44 For example, the energy storage capacity and cycle stability was shown to increase for Fe3O4 on rGO versus pure Fe3O4 or Fe2O3 (Figure 2).43 High surface area rGO has been made using microwaves for exfoliation and reduction of GO. The high surface area rGO formed is useful as an energy storage material in supercapacitors.45,46

energy storage capacity and cycle stability

Figure 2.(a) Discharge/charge profiles of the GNS/Fe3O4 composite. (b) Cycling performance of the commercial Fe3O4 particles, GNS/Fe3O4 composite and bare Fe2O3 particles at a current density of 35 mA/g Solid symbols, discharge; hollow symbols, charge. (c) Cycling performance of the GNS/Fe3O4 composite at a current density of 700 mA/g for 100 cycles. (d) Rate performance of the commercial Fe3O4 particles, GNS/Fe3O4 composite, and bare Fe2O3 particles at different current densities. GNS = rGO. Adapted with permission from reference 43. Copyright 2010 American Chemical Society.

Biomedical Applications

One use of GO in the biomedical field is as a component in drug delivery systems. Functionalized nanographene oxide (nGO, product number 795534) has been used in several studies on targeted delivery of anti-cancer drugs. Polyethylene glycol (PEG) functionalized nGO with SN38, a camptothecin (product number H0165) derivative, adsorbed onto the surface (nGO‒PEG‒SN38) was used to as a water and serum soluble source of the drug.47 In that study, nGO‒PEG‒SN38 was shown to be three orders of magnitude more effective than irinotecan (CPT-11), an FDA approved SN38 prodrug, at reducing the cell viability of human colon cancer cell lines HTC-116 (Figure 3).47 The effectiveness of nGO‒PEG‒SN38 was similar to SN38 in DMSO (Figure 3).47 Melanoma skin cancer in mice has been treated using photothermal ablation therapy with a near-infrared laser and nGO that was functionalized with PEG and hyaluronic acid and delivered transdermally.48 In a separate study, magnetite was adsorbed onto GO loaded with the anti-cancer drug doxorubicin hydrochloride (DXR, product numbers D1515 and 44583) for targeted delivery of the drug to specific sites using magnets.49

In vitro cell toxicity assay

Figure 3.In vitro cell toxicity assay. (a) Relative cell viability (versus untreated control) data of HCT-116 cells incubated with CPT-11, SN38, and NGO‒PEG‒SN38 at different concentrations for 72 h. Free SN38 was dissolved in DMSO and diluted in PBS. Water soluble NGO‒PEG‒SN38 showed similar toxicity as SN38 in DMSO and far higher potency than CPT-11. (b) Relative cell viability data of HCT-116 cells after incubation with nGO-PEG with (red) and without (black) SN38 loading. Plain NGO‒PEG exhibited no obvious toxicity even at very high concentrations. Error bars were based on triplet samples. NGO = nGO. Adapted with permission from reference 47. Copyright 2008 American Chemical Society.


GO and rGO have been used as components in several systems designed to detect biologically relevant molecules. GO has been used as a fluorescence quenching material in biosensors which utilize the fluorescence resonance energy transfer (FRET) effect. In one study by Lu et al., single-stranded DNA (ssDNA) with a fluorescence tag was found to bind noncovalently to GO with subsequent quenching of the fluorescence of the tag.50 Addition of a complementary ssDNA removed the tagged DNA from the GO surface and restored the fluorescence. The FRET effect was also used with a fluorescein labeled ATP aptamer to sense ATP as low as 10 μM.51 Folic acid functionalized GO was used as a component in a system used to detect human cervical cancer and human breast cancer cells.52

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Compton OC, Nguyen ST. 2010. Graphene Oxide, Highly Reduced Graphene Oxide, and Graphene: Versatile Building Blocks for Carbon-Based Materials. Small. 6(6):711-723.
Mao S, Pu H, Chen J. 2012. Graphene Oxide and its Reduction: Modeling and Experimental Progress. RSC Adv.. 22643.
Dreyer DR, Park S, Bielawski CW, Ruoff RS. The chemistry of graphene oxide. Chem. Soc. Rev.. 39(1):228-240.
Novoselov KS. 2004. Electric Field Effect in Atomically Thin Carbon Films. Science. 306(5696):666-669.
Gilje S, Han S, Wang M, Wang KL, Kaner RB. 2007. A Chemical Route to Graphene for Device Applications. Nano Lett.. 7(11):3394-3398.
Chua CK, Pumera M. Chemical reduction of graphene oxide: a synthetic chemistry viewpoint. Chem. Soc. Rev.. 43(1):291-312.
Pei S, Cheng H. 2012. The reduction of graphene oxide. Carbon. 50(9):3210-3228.
Zhu Y, Murali S, Cai W, Li X, Suk JW, Potts JR, Ruoff RS. 2010. Graphene and Graphene Oxide: Synthesis, Properties, and Applications. Adv. Mater.. 22(35):3906-3924.
Chen D, Feng H, Li J. 2012. Graphene Oxide: Preparation, Functionalization, and Electrochemical Applications. Chem. Rev.. 112(11):6027-6053.
Wan X, Huang Y, Chen Y. 2012. Focusing on Energy and Optoelectronic Applications: A Journey for Graphene and Graphene Oxide at Large Scale. Acc. Chem. Res.. 45(4):598-607.
Eda G, Chhowalla M. Chemically Derived Graphene Oxide: Towards Large-Area Thin-Film Electronics and Optoelectronics. Adv. Mater.. 22(22):2392-2415.
Wan X, Long G, Huang L, Chen Y. 2011. Graphene - A Promising Material for Organic Photovoltaic Cells. Adv. Mater.. 23(45):5342-5358.
Huang X, Qi X, Boey F, Zhang H. Graphene-based composites. Chem. Soc. Rev.. 41(2):666-686.
Lightcap IV, Kamat PV. 2013. Graphitic Design: Prospects of Graphene-Based Nanocomposites for Solar Energy Conversion, Storage, and Sensing. Acc. Chem. Res.. 46(10):2235-2243.
Sun X, Liu Z, Welsher K, Robinson JT, Goodwin A, Zaric S, Dai H. 2008. Nano-graphene oxide for cellular imaging and drug delivery. Nano Res.. 1(3):203-212.
Chung C, Kim Y, Shin D, Ryoo S, Hong BH, Min D. 2013. Biomedical Applications of Graphene and Graphene Oxide. Acc. Chem. Res.. 46(10):2211-2224.
Wang Y, Li Z, Wang J, Li J, Lin Y. 2011. Graphene and graphene oxide: biofunctionalization and applications in biotechnology. Trends in Biotechnology. 29(5):205-212.
Pyun J. 2011. Graphene Oxide as Catalyst: Application of Carbon Materials beyond Nanotechnology. Angew. Chem. Int. Ed.. 50(1):46-48.
Yeh T, Syu J, Cheng C, Chang T, Teng H. Graphite Oxide as a Photocatalyst for Hydrogen Production from Water. Adv. Funct. Mater.. 20(14):2255-2262.
Dreyer D, Jia H, Bielawski C. 2010. Inside Cover: Graphene Oxide: A Convenient Carbocatalyst for Facilitating Oxidation and Hydration Reactions (Angew. Chem. Int. Ed. 38/2010). Angewandte Chemie International Edition. 49(38):6686-6686.
Kim J, Cote LJ, Kim F, Yuan W, Shull KR, Huang J. 2010. Graphene Oxide Sheets at Interfaces. J. Am. Chem. Soc.. 132(23):8180-8186.
Hummers WS, Offeman RE. 1958. Preparation of Graphitic Oxide. J. Am. Chem. Soc.. 80(6):1339-1339.
Shin H, Kim KK, Benayad A, Yoon S, Park HK, Jung I, Jin MH, Jeong H, Kim JM, Choi J, et al. 2009. Efficient Reduction of Graphite Oxide by Sodium Borohydride and Its Effect on Electrical Conductance. Adv. Funct. Mater.. 19(12):1987-1992.
Fernández-Merino MJ, Guardia L, Paredes JI, Villar-Rodil S, Solís-Fernández P, Martínez-Alonso A, Tascón JMD. 2010. Vitamin C Is an Ideal Substitute for Hydrazine in the Reduction of Graphene Oxide Suspensions. J. Phys. Chem. C. 114(14):6426-6432.
Moon IK, Lee J, Ruoff RS, Lee H. 2010. Reduced graphene oxide by chemical graphitization. Nat Commun. 1(1):
Pei S, Zhao J, Du J, Ren W, Cheng H. 2010. Direct reduction of graphene oxide films into highly conductive and flexible graphene films by hydrohalic acids. Carbon. 48(15):4466-4474.
Su C, Xu Y, Zhang W, Zhao J, Liu A, Tang X, Tsai C, Huang Y, Li L. 2010. Highly Efficient Restoration of Graphitic Structure in Graphene Oxide Using Alcohol Vapors. ACS Nano. 4(9):5285-5292.
Wang S, Ang PK, Wang Z, Tang ALL, Thong JTL, Loh KP. 2010. High Mobility, Printable, and Solution-Processed Graphene Electronics. Nano Lett.. 10(1):92-98.
Lu G, Park S, Yu K, Ruoff RS, Ocola LE, Rosenmann D, Chen J. 2011. Toward Practical Gas Sensing with Highly Reduced Graphene Oxide: A New Signal Processing Method To Circumvent Run-to-Run and Device-to-Device Variations. ACS Nano. 5(2):1154-1164.
Chen K, Lu G, Chang J, Mao S, Yu K, Cui S, Chen J. 2012. Hg(II) Ion Detection Using Thermally Reduced Graphene Oxide Decorated with Functionalized Gold Nanoparticles. Anal. Chem.. 84(9):4057-4062.
He Q, Wu S, Yin Z, Zhang H. 2012. Graphene-based electronic sensors. Chem. Sci.. 3(6):1764.
He Q, Sudibya HG, Yin Z, Wu S, Li H, Boey F, Huang W, Chen P, Zhang H. 2010. Centimeter-Long and Large-Scale Micropatterns of Reduced Graphene Oxide Films: Fabrication and Sensing Applications. ACS Nano. 4(6):3201-3208.
He Q, Wu S, Gao S, Cao X, Yin Z, Li H, Chen P, Zhang H. 2011. Transparent, Flexible, All-Reduced Graphene Oxide Thin Film Transistors. ACS Nano. 5(6):5038-5044.
Cai B, Wang S, Huang L, Ning Y, Zhang Z, Zhang G. 2014. Ultrasensitive Label-Free Detection of PNA?DNA Hybridization by Reduced Graphene Oxide Field-Effect Transistor Biosensor. ACS Nano. 8(3):2632-2638.
Liu Y, Yu D, Zeng C, Miao Z, Dai L. 2010. Biocompatible Graphene Oxide-Based Glucose Biosensors. Langmuir. 26(9):6158-6160.
Matyba P, Yamaguchi H, Eda G, Chhowalla M, Edman L, Robinson ND. 2010. Graphene and Mobile Ions: The Key to All-Plastic, Solution-Processed Light-Emitting Devices. ACS Nano. 4(2):637-642.
Becerril HA, Mao J, Liu Z, Stoltenberg RM, Bao Z, Chen Y. 2008. Evaluation of Solution-Processed Reduced Graphene Oxide Films as Transparent Conductors. ACS Nano. 2(3):463-470.
Saha SK, Bhaumik S, Maji T, Mandal TK, Pal AJ. Solution-processed reduced graphene oxide in light-emitting diodes and photovoltaic devices with the same pair of active materials. RSC Adv.. 4(67):35493-35499.
Li S, Tu K, Lin C, Chen C, Chhowalla M. 2010. Solution-Processable Graphene Oxide as an Efficient Hole Transport Layer in Polymer Solar Cells. ACS Nano. 4(6):3169-3174.
Wang H, Cui L, Yang Y, Sanchez Casalongue H, Robinson JT, Liang Y, Cui Y, Dai H. 2010. Mn3O4?Graphene Hybrid as a High-Capacity Anode Material for Lithium Ion Batteries. J. Am. Chem. Soc.. 132(40):13978-13980.
Yang S, Feng X, Ivanovici S, Müllen K. 2010. Fabrication of Graphene-Encapsulated Oxide Nanoparticles: Towards High-Performance Anode Materials for Lithium Storage. Angew. Chem. Int. Ed.. 49(45):8408-8411.
Lee JK, Smith KB, Hayner CM, Kung HH. 2010. Silicon nanoparticles?graphene paper composites for Li ion battery anodes. Chem. Commun.. 46(12):2025.
Zhou G, Wang D, Li F, Zhang L, Li N, Wu Z, Wen L, Lu GQ(, Cheng H. 2010. Graphene-Wrapped Fe3O4Anode Material with Improved Reversible Capacity and Cyclic Stability for Lithium Ion Batteries. Chem. Mater.. 22(18):5306-5313.
Zhang M, Lei D, Yin X, Chen L, Li Q, Wang Y, Wang T. 2010. Magnetite/graphene composites: microwave irradiation synthesis and enhanced cycling and rate performances for lithium ion batteries. J. Mater. Chem.. 20(26):5538.
Zhu Y, Murali S, Stoller MD, Ganesh KJ, Cai W, Ferreira PJ, Pirkle A, Wallace RM, Cychosz KA, Thommes M, et al. 2011. Carbon-Based Supercapacitors Produced by Activation of Graphene. Science. 332(6037):1537-1541.
Zhu Y, Murali S, Stoller MD, Velamakanni A, Piner RD, Ruoff RS. 2010. Microwave assisted exfoliation and reduction of graphite oxide for ultracapacitors. Carbon. 48(7):2118-2122.
Liu Z, Robinson JT, Sun X, Dai H. 2008. PEGylated Nanographene Oxide for Delivery of Water-Insoluble Cancer Drugs. J. Am. Chem. Soc.. 130(33):10876-10877.
Jung HS, Kong WH, Sung DK, Lee M, Beack SE, Keum DH, Kim KS, Yun SH, Hahn SK. 2014. Nanographene Oxide?Hyaluronic Acid Conjugate for Photothermal Ablation Therapy of Skin Cancer. ACS Nano. 8(1):260-268.
Yang X, Zhang X, Ma Y, Huang Y, Wang Y, Chen Y. 2009. Superparamagnetic graphene oxide?Fe3O4 nanoparticles hybrid for controlled targeted drug carriers. J. Mater. Chem.. 19(18):2710.
Lu C, Yang H, Zhu C, Chen X, Chen G. 2009. A Graphene Platform for Sensing Biomolecules. Angew. Chem. Int. Ed.. 48(26):4785-4787.
Wang Y, Li Z, Hu D, Lin C, Li J, Lin Y. 2010. Aptamer/Graphene Oxide Nanocomplex forin SituMolecular Probing in Living Cells. J. Am. Chem. Soc.. 132(27):9274-9276.
Song Y, Chen Y, Feng L, Ren J, Qu X. 2011. Selective and quantitative cancer cell detection using target-directed functionalized graphene and its synergetic peroxidase-like activity. Chem. Commun.. 47(15):4436.
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