The concentration can be confirmed on the Certificate of Analysis that's specific to the lot you received, found here:https://www.sigmaaldrich.com/US/en/product/sigma/p2980#product-documentation
As of April 2024, the current specification is 0.2mg/mL
P2980
Pertussis toxin
from Bordetella pertussis, buffered aqueous glycerol solution, protein endotoxin
Pertussis toxin from Bordetella pertussis
Synonym(s):
Histamine-sensitizing factor, IAP, Islet Activating Protein, PTX, Pertussigen
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P2980-50UG
R 10,413.48
P2980-.2MG
R 34,758.64
R 10,413.48
List PriceR 10,626.00Please contact Customer Service for Availability
About This Item
Form:
buffered aqueous glycerol solution
Quality level:
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Product Name
Pertussis toxin from Bordetella pertussis, buffered aqueous glycerol solution
SMILES string
O(C)C(=O)CCCCCCCCCCCCCCCCCCCCCCCC
InChI
1S/C26H52O2/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20-21-22-23-24-25-26(27)28-2/h3-25H2,1-2H3
InChI key
WOPKHAQDUMDJIY-UHFFFAOYSA-N
description
aseptically packaged
form
buffered aqueous glycerol solution
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
Bordetella pertussis Tohama I ... ptxA(2665068), ptxB(2665069), ptxC(2665408)
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Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| form buffered aqueous glycerol solution | form lyophilized powder | form powder | form buffered aqueous solution |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in - | shipped in - | shipped in dry ice |
| description aseptically packaged | description - | description - | description - |
| Gene Information Bordetella pertussis Tohama I ... ptxA(2665068), ptxB(2665069), ptxC(2665408) | Gene Information Bordetella pertussis Tohama I ... ptxA(2665068), ptxB(2665069), ptxC(2665408) | Gene Information human ... IAPP(3375) | Gene Information human ... PITX1(5307) |
General description
Pertussis (Pertussis) toxin (PTX) is a protein isolated from the bacterium Bordatella (Bordatella) coccobaccillus. The toxin is excreted in a non-active state. When the B oligomer of the bacterium, Bordatella coccobacillus, comes in contact with the cell membrane, an S1 subunit of its A protomer is activated. This activation may be mediated by glutathione and/or ATP.
Application
Inactive toxin form released from B. pertussis.
Pertussis toxin from Bordetella pertussis has been used: to enhance cardiac myosin immunization of rats to induce myocarditis[1]; to break down the blood–brain barrier in the Cuprizone and cuprizone/MOG-EAE model;[2] as an inhibitor of Gαi signaling[3]
The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al.
Biochem/physiol Actions
Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt.
Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels.
Features and Benefits
This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
Physical form
Aseptically filled solution in 50% glycerol, containing 50 mM TRIS, 10 mM glycine, 0.5 M NaCl, pH 7.5
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Instructions
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