Mammalian cell culture

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Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.

Cell Culture Protocol 10: Testing Cells for Mycoplasma Contamination by Hoechst DNA Staining

Cell culture protocol for testing cell lines for mycoplasma contamination using indirect DNA staining using Hoechst 33342.

NF-κB and Inflammation

Chronic inflammation is an underlying factor in the development and progression of many of the chronic diseases of aging, such as arthritis, atherosclerosis, diabetes, and cancer.

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

Cell culture protocol for passaging and splitting suspension cell lines

Hepatocyte Growth Factor (HGF)

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.

L-15 Media Formulation

Alternative High-Yield Cell Culture Systems

Solutions for high-yield cell culture scale-up applications including multi-layered flasks (HyperFlasks/CellStacks), roller bottles, spinner and shaker flasks. Free ECACC handbook download.

F-12 Coon's Modification Formulation

Cell Analysis Best Practices

How do you assess the state of your cell culture? We’ve got some best practices to share.

Hybridoma Fusion Partners Cell Lines

Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.

Common Cell Culture Problems: Precipitates

Common Cell Culture Problems: Precipitates -- When contamination is ruled out, turbidity in cell culture media is often explained by the precipitation of metals, proteins, and other media components. Precipitates can be harmful to cell health, as they may alter

Cultivating Consistency: Tips and Tricks for Successful Cell Cultures

Properly optimizing workflows and utilizing the latest best practices are the keys to obtaining consistent results and advancing research.

Lipoic (Thioctic) Acid, in Cell Culture

How lipoic acid (thioctic acid) and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

Oleic Acid in Cell Culture

How the unsaturated fatty acid, oleic acid and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

Palmitic Acid in Cell Culture

How palmitic acid, other fatty acids and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

Copper in Cell Culture

Importance and uses of copper in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO), cell cultures

Citrate Serum-Free Medium Supplement

Citrate is one of many cell culture components that affects the performance of serum-free, protein-free and animal protein-free cell culture systems used for biomanufacturing heterologous proteins such as monoclonal antibodies.

BRAND® Insert 2in1 Supports the Cultivation of Reconstructed Human Epidermis (RhE) used for Skin Corrosion Tests (OECD TG 431)

The BRAND® Insert 2in1 cell culture insert supports the cultivation of Reconstructed Human Epidermis used for skin corrosion tests.

Autophagy in Cancer Promotes Therapeutic Resistance

We presents an article on Autophagy in Cancer Promotes Therapeutic Resistance

Insulin-like Growth Factors (IGF)

Lipid-induced Insulin Resistance

Lipid Induced Insulin Resistance

Cell Freezing and Banking

Removal of Percoll after Centrifugation

This page shows methods to remove Percoll from a sample after centrifugation.

Cell Death Troubleshooting in Cell Culture

Here we discuss causes of cell death, and how to assess and prevent these causes.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

DMEM/nutrient Mixture F-12 Formulation

Dulbecco's Modified Eagle's Medium/Ham's Nutrient Mixture F-12 (DME/F12) Formulation

Cell Line Cross-Contamination and Misidentification; Common Cell Culture Problems

Cell line cross-contamination and misidentification can be solved with authentication and conscientious sourcing.

Ascorbate in Cell Culture

Importance and uses of ascorbate in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Ferric and Ferrous Iron in Cell Culture

Importance and uses of ferric and ferrous iron in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Glucose in Cell Culture

Importance and uses of glucose in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

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