Protein expression

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Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL

Protein synthesis using cell-free protein synthesis reagent kit consists of three stages: preparation of transcription template, transcription and translation.

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

Yale Immunofluorescence Protocol

Procedure for Fixation, Immunostaining, and Imaging in 384-well Plates.

Duolink® PLA Fluorescence Protocol

This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.

ALiCE®Cell-Free Protein Synthesis System Protocol

Achieve high protein yields with the ALiCE® Cell-Free Protein Synthesis System that allows you to obtain “difficult to produce” proteins in a matter of hours instead of weeks.

Liberase™ TM Research Grade Protocol & Troubleshooting

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

cOmplete™ Protocol

It is possible dissolve 1 tablet in as little as 2 ml double distilled Water, results in a 25x stock solution, without difficulty.

AliCE® Cell-Free Protein Synthesis Kit Quick Start Guide

Follow these five quick steps to perform cell-free protein synthesis using the ALiCE® protein expression plant lysate kit.

Duolink® PLA Multicolor Detection Protocol

Duolink® PLA Probemaker Guide

Guide to create custom PLA probes for immunofluorescent or brightfield detection.

Handling Inclusion Bodies in Recombinant Protein Expression

How to isolate proteins from inclusion bodies using Cytiva products.

Counterstaining after the Duolink In Situ Protocol

We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.

Duolink® In Situ Short Instructions - Fluorescence

This page details the Duolink® In Situ Short Protocol for fluorescence detection

Using Duolink® in Protein Interaction Studies

The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.

Measuring Kinase Inhibitor Residence Times

Jump dilution protocol to determine the residence time of a drug (or drug candidate) during its interaction with a kinase using a fluorescence polarization assay based on the detection of ADP.

Creating PLA® Probes

Duolink® kits use in situ PLA® technology for accurate quantification of individual proteins, interactions, and modifications in cells and tissue.

Duolink® PLA Brightfield Protocol

Duolink® PLA reagents enable brightfield detection and quantification of proteins and interactions in tissue samples.