Purification

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Showing 1-29 of 29 results

FastWoRX for Faster and Greener Alternative to Liquid-Liquid Extraction

Description of viral clearance using Capto adhere from Cytiva.

Maintenance of Multimodal Chromatography Media and Storage Conditions

This page describes the maintenance of media and storage conditions for multimodal chromatography using Cytiva products.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Capto MMC

Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Purification or Removal of Viruses including Adeno-associated Virus

This page shows how to purify or remove viruses with a Capto DeVirS, AVB Sepharose High Performance from Cytiva.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Chiral Chromatography Frequently Asked Questions

Hydrophobic Interaction Chromatography Setup

Column, media, and sample preparation for Hydrophobic Interaction Chromatography (HIC) detailed with Cytiva media.

Troubleshooting Reversed Phase Chromatography

“Ghosting” in chromatograms due to poor eluent quality; trace organic impurities concentrate, causing unknown peaks.

High Purity Carbon Adsorbents for Chromatography

Synthetic CMS carbons offer tailored adsorbents for specific applications.

Polishing of MAbs Using Capto Adhere ImpRes in Bind/Elute Mode

In these studies, the binding capacity for MAbs and the efficiency in the clearance of impurities using Capto adhere ImpRes in bind/elute mode was evaluated.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Performing a Purity and Homogeneity Check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

Bind/Elute vs Flowthrough Mode in Multimodal Chromatography

This page describes the difference between bind/elute and ﬂowthrough modes in multimodal chromatography using Cytiva products.

Capto Adhere Anion Exchanger for MAbs & Bind/Elute

Capto adhere from Cytiva is a multimodal strong anion exchanger for BioProcess applications.

Protein G and Protein A Bind to Different IgG

This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

LRA (Lipid Removal Agent) for Biopharmaceutical Production

Lipids are naturally-occurring hydrocarbons that are poorly soluble in water and soluble in non-polar solvents such as ether and chloroform.

Solvent Miscibility Table

Substances are said to be miscible in one another if they dissolve to form a uniform solution. Bookmark or download our miscibility table for common lab solvents.

Immobilized Metal Chelate Affinity Chromatography (IMAC)

How to separate proteins and peptides with afﬁnity for metal ions by immobilized metal chelate afﬁnity chromatography.

Plasmid purification by ion exchange chromatography with Genopure kits

Ion exchange chromatography is employed by the Genopure Plasmid Midi Kit and the Genopure Plasmid Maxi Kit.

Setting Pressure Limits for Size Exclusion Chromatography

Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.

Protein Affinity Chromatography

Affinity chromatography purifies compounds using selective ligands on porous matrix, ensuring specific recovery.