Chemical Standards

Glycerin determination by ASTM D6584 and/or DIN EN 14105 requires the use of two internal standards and multi-component calibration solutions, each containing glycerin, monoolein, diolein, and triolein, in varying concentrations. Analysts following ASTM D6584 are required to prepare five different multi-component solutions, while those adhering to DIN EN 14105 must prepare only four different multi-component solutions. Although these chemicals are readily available, the preparation can be time consuming and requires working with pyridine.

Calibration standards preparation for both methods is made easier with pre-made, multi-component, varied concentration solutions. Use of these standards saves time and reduces exposure to pyridine. Step-by-step preparation instructions are included, and were purposefully developed to be similar to those used in preparing B100 biodiesel samples. This ensures that error, due to variation in the preparation of samples vs. standards, will be minimized.

Internal Standards

ASTM D6584 and DIN EN 14105 both specify the use of two internal standards, butanetriol and tricaprin. Both of these internal standards are added to every B100 biodiesel sample prior to analysis. They are used to compensate for run-to-run injection variations through comparison to the internal standard retention times and levels in the calibration standards.

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CRM44896 ASTM® D6584 1,2,4-Butanetriol Solution certified reference material, 1000 μg/mL in pyridine, ampule of 5 mL
44897-U ASTM® D6584 Tricaprin Solution 8000 μg/mL in pyridine, analytical standard Green Alternative
CRM44897 ASTM® D6584 Tricaprin Solution certified reference material, 8000 μg/mL in pyridine

Method ASTM D6584 Calibration Standard Mixes and Kit

Preparation of ASTM D6584 calibration standards involves transferring a known amount of each of our five standard solutions into separate vials along with both internal standards (butanetriol and tricaprin) and MSTFA silylation reagent. After allowing the standard mixtures 20 minutes to fully derivatize, each is diluted with n-heptane. The standards are now ready for GC analysis.

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44914-U ASTM® D6584 Standard Solution 2 in pyridine (varied), analytical standard Green Alternative
44916-U ASTM® D6584 Standard Solution 4 in pyridine (varied), analytical standard Green Alternative

Method ASTM D6584 Individual Calibration Standards and Kit

We offer each ASTM D6584 analyte as an individual reference material for those who wish to prepare their own calibration standards.

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44894-U ASTM® D6584 1,3-Diolein Solution 5000 μg/mL in pyridine, analytical standard Green Alternative
44892-U ASTM® D6584 Glycerin solution 500 μg/mL in pyridine, analytical standard Green Alternative
44893-U ASTM® D6584 Monoolein Solution 5000 μg/mL in pyridine, analytical standard Green Alternative
CRM44895 ASTM® D6584 Triolein solution certified reference material, 5000 μg/mL in pyridine, ampule of 1 × 2 mL

Method EN 14105 Monoglyceride Stock Solution

Due to the possible overlap of FAME and derivatized monoglyceride peaks in the chromatographic analysis, DIN EN 14015 also recommends use of a monoglyceride mixture containing monopalmitin, monolein, and monostearin to aid in peak identification. We offer a monoglyceride stock solution that can be used for this purpose.

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49446-U EN 14105/D6584 Monoglyceride Stock Solution 10 mg/mL each component in pyridine, analytical standard Green Alternative