Free Fatty Acids

Short chain, volatile fatty acids are typically analyzed in the free form using specialized columns. This group of compounds may be referred to as free fatty acids (FFAs), volatile fatty acids (VFA), or carboxylic acids. The analysis of fatty acids in the free form instead of as fatty acid methyl esters results in easier and quicker sample preparation. Additionally, artifact formation that may result from a derivatization procedure is eliminated.

For the GC analysis of free fatty acids, a specialized column that will not allow the adsorption of active carboxyl groups is required. The Nukol, with its acidic characteristic, is well-suited for this application, allowing chromatography with excellent peak shapes.

Technical Article:
GC Analyses of Free Fatty Acids