Fatty acids

It is important to use only high quality reagents in derivatization of fatty acids to FAMEs to ensure that no artifacts are introduced during the processing of samples. Additionally, only derivatization reagents with low moisture should be used, as the esterification reaction will be hindered by the presence of water. The storage conditions of all reagents should be strictly adhered to, as some are susceptible to degradation during long-term storage.

Fatty Acids to FAME Derivatization

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33126-U BF3 - Butanol solution 10 % (w/w)
33356 BF3 - Methanol 10 % (w/w), pkg of 20 × 1 mL
33355 Methanolic HCl 3 M HCl in methanol (3N), for GC derivatization, ampule of 20 × 1 mL

Water Scavenger

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D136808 2,2-Dimethoxypropane reagent grade, 98%

Drying

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239313 Sodium sulfate ACS reagent, ≥99.0%, anhydrous, granular