Biomolecules and the matrixes in which they are analyzed are often complex. To accommodate the complexity and maintain biological activity, if required, many different HPLC modes can be employed. We have chosen to include in our product offering select columns and media from the major separation modes.

IR and UV techniques can also be used to determine protein content. This is because various functional groups adsorb energy at specific wavelengths. For example, monitor the energy absorbed at IR bands characteristic of the peptide bond (3300-3500 nm, 2080-2220 nm, and 1560-1670 nm), or the energy absorbed at UV 280 nm (characteristic of tryptophan and tyrosine residues). These methods are attractive due to being rapid, non-destructive, and requiring limited sample preparation. Calibration curves are necessary for proper measurement.

Technical Article:
Separation of Proteins and Peptides on Discovery BIO Wide Pore (300 Å) C18, C8, and C5 Phases
High-Efficiency Ion-Exchange Separations of Proteins and Peptides on Discovery BIO PolyMA