GE Life Sciences Gel Filtration Columns

Gel filtration chromatograhy of proteins was first practiced in 1959 by Jerker Porath and Per Flodin at Uppsala University and was subsequently commercialized by Pharmacia Fine Chemicals under the trade name Sephadex®. In subsequent years, a continuous stream of improvements and innovations in gel filtration chromatography were introduced by Pharmacia and, as it was later called, Amersham Biotech and now GE Life Sciences, a division of GE Healthcare. Gel filtration packing materials that have become household names for generations of biochemists include the trade names Sephacryl®, Superose® and Superdex® and column formats such as HILoad®, HiPrep, Tricom, XK and Precision Column. All columns from GE Life Science can be used in FPLC and selected AKTA liquid chromatography systems.

Products in this section are listed by the brand names that have been popularized by GE Life Sciences. Sephacryl, Sephadex, Superdex and Superose. In general, Superdex Increase columns are most efficient as they are packed with 8.6 micron particles, while Superose (and Sephacryl) columns have broader fractionation ranges. Of the preparative columns, Sephacryl HiPrep columns are available in five pore size ranges but are packed with larger particles than the more efficient Superdex HiLoad columns. Sephadex Desalting columns exclude proteins with molecular weights above 5000 dalton and allow salts to elute after the protein peak.
Two Gel Filtration Calibration Standards are available for calibration or to check column performance. Although each protein standard can be used with other types of gel filtration columns, Sigma-Aldrich does not recommend to inject Blue Dextran on silca-based gel filtration columns.