TLC Plates, Glass backing

Glass is the most common plate backing because it is rigid, transparent and chemically resistant to all mobile phases and visualization methods, most notably to charring with corrosive agents. Glass plates are also reusable; an important consideration for analysts who make their own plates. For in situ densitometric quantification, the use of highly uniform pre-coated layers on glass is virtually mandatory for adequate reproducibility and accuracy. Scored glass plates are also available that can be easily snapped into smaller, more convenient sizes to accommodate fewer samples or smaller developing tanks.