HybridSPE®-Phospholipid Technology

Effective Removal of Phospholipids and Proteins for Accurate and Reproducible LC-MS Analyses

Key Features
  • Simple methodology, analogous to standard protein precipitation
  • Available in both 96-well plate and cartridge formats (see product list below)
  • Unique chemistry for excellent recovery of both hydrophobic and hydrophilic analytes
  • Part of our bioanalytical LC-MS workflow solution
Reduce Overall Cost per Analysis, Increase Throughput and Improve LC-MS Data Consistency

Product overview video

Visit the HybridSPE home page to learn more about:
New! HybridSPE-PLus plates; the basics of HybridSPE methodology; impact of phospholipids on LC-MS results; and the use of HybridSPE technology for phospholipid enrichment
HybridSPE-PLus 96-Well Plate
HybridSPE-Phospholipid Ultra Cartridges

HybridSPE® Plates

96-well plates are an excellent choice for higher-throughput analyses. We offer HybridSPE-PLus plates, suitable for sample volumes between 100 and 300 μL; as well as our Small Volume HybridSPE-Phospholipid plates, suitable for sample volumes between 20 and 40 μL, in a 96-well plate format. Plates are sold individually as well as in bulk packs of 20 plates.

For analysts who are new to the technology, we also offer a HybridSPE-PLus Plate Essentials Kit (Part number 52818-U) that includes one 96-well HybridSPE-PLus plate, one plate cap mat, one sealing film and a collection plate.

Product #

Description

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52794-U HybridSPE®-Phospholipid Small Volume 96-well Plate, bed wt. 15 mg, volume 0.8 mL, pk of 1
52798-U HybridSPE®-Phospholipid Small Volume 96-well Plate, bed wt. 15 mg, volume 0.8 mL, pk of 20
575659-U HybridSPE®-PLus 96-Well Plate bed wt. 50 mg, volume 2 mL, pk of 1 ea
575673-U HybridSPE®-PLus 96-Well Plate bed wt. 50 mg, volume 2 mL, pk of 20 ea
52818-U HybridSPE®-PLus 96-Well Plate Essentials Kit 96-Well Essentials Kit, pk of 1
55263-U 96-Well Protein Precipitation Filter Plate pk of 1

HybridSPE® Plate Accessories

Product #

Description

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Z721581 EZ-Pierce films non-sterile, Excel Scientific, EZP-100
Z717266 Nunc® 96 DeepWell plate, non-treated maximum volume 2 mL, non-sterile, natural polypropylene wells round bottom
57192-U PlatePrep 96-well Vacuum Manifold Manifold only
575680-U 96 Round Well Pierceable Cap Mats for use with HybridSPE-PLus 96-well plates, pk of 50 ×

HybridSPE® Cartridges

Cartridges allow for easy processing of just one or a few samples at a time. Like our 96-well plate products, the 1 mL HybridSPE-Phospholipid Ultra cartridge is capable of removing both proteins and phospholipids in an online format. If an offline protein precipitation is preferred, the 1 mL or 6 mL HybridSPE-Phospholipid cartridges, which do not contain a protein precipitation filter, can be used for phospholipid removal following a separate offline protein precipitation (using a product such as Cat. No. 55263-U, 96-well protein precipitation filter plate to remove precipitated proteins from the sample first).

Product #

Description

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55261-U HybridSPE®-Phospholipid Cartridge, bed wt. 30 mg, volume 1 mL, pk of 100
55276-U HybridSPE®-Phospholipid Cartridge, bed wt. 30 mg, volume 1 mL, pk of 200, polypropylene material (hardware), PE frit (20 μm)
55267-U HybridSPE®-Phospholipid Cartridge, bed wt. 500 mg, volume 6 mL, pk of 30
55269-U HybridSPE®-Phospholipid Ultra cartridge, bed wt. 30 mg, volume 1 mL, pk of 100

HybridSPE® Cartridge Accessories

Product #

Description

Add to Cart

57059 Disposable Liners for Visiprep DL Manifolds (included with 57044 and 57265) PTFE, pk of 100
57044 Visiprep SPE Vacuum Manifold DL (Disposable Liner), 12-port model
57265 Visiprep SPE Vacuum Manifold DL (Disposable Liner), 24-port model
57030-U Visiprep SPE Vacuum Manifold standard, 12-port model
57250-U Visiprep SPE Vacuum Manifold standard, 24-port model

HybridSPE® DPX Tips

DPX stands for Disposable Pipette EXtraction, a patented solid-phase extraction device that is unique from other SPE devices. These pipette tips incorporate loosely contained HybridSPE sorbent material, which is mixed with the sample solution via aspirating and dispensing. The tips can be used manually with single or multi-channel pipettors, or on an automated platform.

Product #

Description

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52973-U HybridSPE® DPX Tip weight 30 mg (bed), Tecan, tip volume 200 μL New
52974-U HybridSPE® DPX Tip weight 50 mg (bed), Tecan, tip volume 1 mL New
52977-U HybridSPE® DPX Tip weight 30 mg (bed), Hamilton, tip volume 300 μL New
52978-U HybridSPE® DPX Tip weight 50 mg (bed), Hamilton, tip volume 1 mL New
52979-U HybridSPE® DPX Tip weight 30 mg (bed), INTEGRA, tip volume 300 μL New
52980-U HybridSPE® DPX Tip weight 50 mg (bed), INTEGRA, tip volume 1.250 mL New
52981-U HybridSPE® DPX Tip weight 30 mg (bed), Universal format, tip volume 1 mL New
52982-U HybridSPE® DPX Tip weight 50 mg (bed), Universal format, tip volume 1 mL New

Precipitation Solvents, Blends, Additives

Product #

Description

Add to Cart

251275 Citric acid ACS reagent, ≥99.5%

Related Equipment

In addition to the related equipment listed below, we also offer a variety of KNF Laboport® Vacuum Pumps. Please inquire for details.

Product #

Description

Add to Cart

Z654760 IKA® VORTEX 3 vortex shaker, 115V, 1/cs
Z654779 IKA® VORTEX 3 vortex shaker, 230V, 1/cs
57161-U SPE Manifold Gauge/Bleed Valve Remote In-Line Design
57120-U SPE Vacuum Pump Trap Kit