Ascentis® Express 2 µm UHPLC Columns

An optimized solution for high throughput small molecule analysis
Features of 2 µm Fused-Core® Particle
  • Outperforms other smaller sub-2 µm particles in efficiency and addresses the disadvantages inherent in smaller sub-2 µm UHPLC columns
  • Delivers perfect balance of pressure and performance ideal for faster small molecule analysis on UHPLC systems

On-demand webinar:
Efficient Separation in UHPLC
Using 2.0 µm Fused-Core Columns

Ordering Information
C18, F5, OH5, HILIC, C8,
Phenyl-Hexyl, RP-Amide, ES-Cyano

Ascentis Express 2 µm
Flyer (view)

Technical Article: Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles
Excellent Peak Shape, Efficiency, and Speed Compared to Fully Porous Particle

1. Oxazepam
2. Nordiazepam
3. Temazepam
4. Diazepam

column:  Ascentis Express C18 ,10 cm x 2.1 mm I.D., 2.0 µm (50813-U)
mobile phase:  (A) water with 0.1% formic acid;
(B) acetonitrile with 0.1% formic acid; (65:35, A:B)
flow rate:  0.8 mL/min
temp.:  35 °C
det:  UV, 240 nm
injection:  0.5 µL
sample:  50 µg/mL in 80:20, water:methanol
instrument:  Dionex Ultimate 3000

Plates per Pressure Comparison Against Core-Type or Solid-Core sub-2 µm UHPLC Columns

column:  5 cm x 2.1 mm I.D.
mobile phase:  A:acetonitrile; B:water; (50:50 – 47:53, A:B)
flow rate:  0.5 mL/min
temp.:  35 °C
instrument:  Shimadzu Nexera

Columns based on Ascentis Express 2 µm particles produce 30% higher efficiency per unit pressure than UHPLC columns based on smaller sub-2 µm solid core particles.

Ascentis Express 2.0 µm Fused-Core Silica Characteristics
Pore Size 90 Å
Max Temp. 60 °C
Pressure 1000 bar (14500 psi)
Features Endcapped
Operating pH Range 2-9
Surface Area 120 m2/g

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.