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BIOshell™ UHPLC and HPLC Columns

Maximum Resolution for Biomolecules

BIOshell™ HPLC and UHPLC columns deliver maximum speed and efficiency for the separation of biomolecules on both HPLC and UHPLC systems. The novel, superficially porous silica particles (SPP), with pore sizes from 90 Å up to 1000 Å, allow the separation of glycans as well as of very large proteins. In particular, a pore size of 1000 Å shows clear advantages over common 300 Å pores for the separation of very large proteins in biotherapeutic drug development such as monoclonal antibodies (mAbs) or proteins with molecular weights greater than 100 kDa.

BIOshell™ HPLC and UHPLC columns are based on Fused-Core® particles in different particle sizes, core and shell dimensions and pore sizes to meet your individual needs:

High-resolution separation of biomolecules with BIOshell™ Fused-Core™ particles

Superior Efficiency
in UHPLC

2.0 µm SPP particle with 160 Å pores. Ideal for the ultra-fast separation of peptides in UHPLC.

 

 

UHPLC Performance with
HPLC Back Pressures

2.7 µm SPP particle with 90 Å, 160 Å and 1000 Å pores. Ideal for the separation of glycans, peptides and large proteins.

Minimal Mass Transfer
Effects for Enhanced Performance

3.4 µm SPP particle with 400 Å pores. Ideal for separation of proteins. 0.2 µm porous shell minimizes band broadening due to mass transfer effects.

 

Enhanced Performance
in HPLC

5 µm SPP particle with 160 Å pores. Ideal for the separation of peptides on standard HPLC instruments

BIOshell™ U/HPLC and HPLC Column Selection

A wide range of BIOshell™ HPLC and U/HPLC columns, with its range of particle sizes, pores and column chemistries, allows the perfect selection of a column to maximize the chromatographic performance of your next biomacromolecule separation.

Guide to selecting BIOshell™ UHPLC and HPLC column phases90a-glycan160a-peptide-c18 160a-phenyl-hexyl 160a-peptide-cn 400a-protein-c18 400a-protein-c4 lgg-1000a-c4 lgg-1000a-c18 lgg-1000a-diphenyl

 

Bonded Phase Chemistry USP Designation Chromatographic Properties / Use Particle Sizes (µm) Pore Size (Å)
C4 Dimethylbutyl L26 High resolution U/HPLC separation of monoclonal antibodies (mAbs), mAb variants, and high molecular weight proteins
2.7 1000
C18 Diisobutyloctadecyl L1 High resolution separation of monoclonal antibodies (mAbs), mAb variants, and other, high molecular weight, hydrophobic proteins 2.7 1000
Diphenyl Diphenylmethyl L11 High resolution U/HPLC separation of monoclonal antibodies (mAbs), mAb variants, and other large proteins with orthogonal selectivity to alkyl-bonded phases 2.7 1000
C4 Dimethylbutyl L26 High resolution U/HPLC separation of monoclonal antibodies, proteins and polypeptides with good peak shape and recovery 3.4 400
C18 Diisobutyloctadecyl L1 High resolution U/HPLC separation of hydrophobic proteins with enhanced performance due to minimal band broadening from mass transfer effects
3.4 400
C18 Diisobutyloctadecyl L1 High efficiency separation of peptides and demonstrated, comprehensive peptide mapping coverage of digested proteins 2, 2.7, 5 160
CN Diisopropylcyanopropyl L10 High efficiency separation of peptides and orthogonal selectivity to alkyl-bonded phases due to pi-pi interactions 2.7, 5 160
Phenyl-Hexyl Dimethylphenyl-hexyl L11 High efficiency separation of peptides with orthogonal selectivity to alkyl-bnded phases due to rigid aromatic ring (shape selectivity) 2.7 160
Glycan (OH5) Penta-hydroxy L95 High resolution U/HPLC separation of glycans, glycopeptides, polar peptides and isobaric oligosaccharides 2.7 90

All BIOshell™ columns are compatible with LC/MS instrumentation as they have been extensively tested for column bleed and exhibit near zero bleeding.

 

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