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[](https://www.sigmaaldrich.com/US/en/applications/analytical-chemistry/liquid-chromatography/small-molecule-hplc) [Small Molecule HPLC](https://www.sigmaaldrich.com/US/en/applications/analytical-chemistry/liquid-chromatography/small-molecule-hplc) Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications). [](https://www.sigmaaldrich.com/US/en/applications/analytical-chemistry/liquid-chromatography/large-molecule-hplc) [Large Molecule HPLC](https://www.sigmaaldrich.com/US/en/applications/analytical-chemistry/liquid-chromatography/large-molecule-hplc) High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure. [](https://www.sigmaaldrich.com/US/en/applications/analytical-chemistry/liquid-chromatography/low-pressure-liquid-chromatography) [Low Pressure Liquid Chromatography](https://www.sigmaaldrich.com/US/en/applications/analytical-chemistry/liquid-chromatography/low-pressure-liquid-chromatography) Low pressure liquid chromatography (LPLC) is a chromatographic technique that operates at low pressures to drive the mobile phase onto the column containing a stationary phase by the action of a pump. Top __Sign In To Continue__ To continue reading please sign in or create an account. 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