Enzyme Confirmation Cards


A quick and easy confirmation of bacteria is always recommended at the end of a bacteria detection process.

Confirmation steps are really important in microbiology, and there is a wide range of methods that can be employed. Often another differential medium or method is used, such as a biochemical or immunological test. In most cases, it is important that the test be performed within a short time in order to release the results as soon as possible, and it should not be too costly. Therefore, Sigma-Aldrich focused their effort on bacteria specific enzymes and a simple detection system with specific chromogenic and fluorogenic substrates. Test cards were developed, where the reaction is visible to the naked eye, and the result is obtained within 10 minutes. A nice product range has been provided to confirm E. coli, Salmonella, Neisseria gonorrhoea, Staphylococcus aureus, Enterococci, Group A Streptococci, Total coliform, Fecal coliform and Gram negatives.
These tests utilize a specific substrate which, when hydrolyzed by a specific enzyme of the target organism (during peptide hydrolysis), produces a blue/white fluorescence, or purple/blue color upon the addition of Reagent B (color developer) when applicable.