qPCR Kits

QPCRScan for Qualitative Detection of Food Pathogens like Salmonella, Listeria and Campylobacter by Real-Time PCR Method

The QPCRScan portfolio is used for the qualitative detection of several food pathogens by Real-Time PCR method. The kits are based on specific primer sets and fluorescence-labeled probes. Flexibility in the use of all common PCR instruments is possible.

The PCR method used for QPCRScan is based on a duplex system (internal amplification control) with specific primers and fluorescence-labeled probes, and utilizes the patented Rehybridization Probe System (RPS) technology. Using this novel probe technique, the method is characterized by the system allowing a homogenous and high-throughput nucleic acid detection that can be automated. Two probes, oligonucleotides, are used; at the 3′ end of one probe is the acceptor and at the other probe, on the 5′ end, a donor is linked. The probes are selected so that they are partially complementary to each other, and the Förster resonance energy transfer (FRET) effect does occur if they are hybridized during the fluorescence measurement at a temperature which is lower than the annealing temperature of primers and probes. During the amplification step, the quencher labeled probe is hybridized with the target DNA and can be destroyed by the exonuclease activity of the Taq-Polymerase. Therefore, the appropriate donor-labeled probe is not quenched during the next measurement.

Figure 1: Principle of Rehybridization Probe System (RPS) technology

The probes specified for the pathogen of interest are labeled with the fluorophore FAM (Carboxyfluorescein; ?max 494 nm excitation, ?max 518 nm emission), while probes for the internal control are labeled with the fluorophore HEX (hexachloro-fluorescein, ?max 535 nm excitation, ?max 556 nm emission).
Any PCR instrument which can measure these two dyes can be used, which makes the system flexible to use.

This method offers the following advantages:

  • Method is very sensitive (1-10cfu)
  • Economic format for 96 well microtiter plate
  • Flexible use of any PCR instrument capable of detecting FAM and HEX
Principle of the Rehybridization Probe System Technology used for the QPCRScan.

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05829 QPCRScan Campylobacter spp. for microbiology
06238 QPCRScan Listeria monocytogenes for microbiology
06040 QPCRScan Listeria spp. for microbiology
91289 QPCRScan Salmonella spp. for microbiology