Selective & Differential Media for Escherichia coli & Coliforms

Escherichia coli (E. coli) & Coliforms

Our selection includes several MacConkey agars and broths for selective differentiation of Escherichia coli (E. coli).

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Selective vs differential media (with examples)
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16016 BRILA MUG Broth for microbiology, NutriSelect Basic   In the detection of E. coli and coliforms. Bile and brilliant green extensively inhibit the growth of accompanying flora, in particular gram-positive microorganisms. The presence of E. coli results in fluorescence in the UV. A positive indole test and possibly gas formation from lactose fermentation provide confirmation.
The broth can be used in conjunction with the MPN method for E. coli and coliform enumeration in the water of bathing areas.
81938 Coliform ChromoSelect Agar for microbiology, NutriSelect Plus Coliform Agar ChromoSelect Coliform ChromoSelect Agar is a selective chromogenic medium recommended for simultaneous detection of Escherichia coli and total coliforms in water and food samples.
85927 ECC ChromoSelect Selective Agar for microbiology, NutriSelect Plus E. coli-Coliform Selective Agar ChromoSelect ECC Selective Agar ChromoSelect ECC ChromoSelect Selective Agar is a selective medium recommended for the simultaneous detection of Escherichia coli and coliforms in water and food samples. The ingredients help even the sublethally injured coliforms to grow rapidly, Tergitol inhibits Gram-positive as well as some Gram-negative bacteria other than coliforms. The chromogenic mixture contains two chromogenic substrates as Salmon-GAL and X-glucuronide. The enzyme β-D-galactosidase produced by coliforms cleaves Salmon-GAL, resulting in the salmon-to-red coloration of coliform colonies. The enzyme β-D-glucuronidase produced by E. coli, cleaves X-glucuronide. E. coli forms dark blue-to-violet colored colonies due to cleavage of both Salmon-GAL and X-glucuronide. The addition of tryptophan improves the indole reaction. To confirm E. coli, add a drop of Kovac′s reagent (Catalog No. 60983) on the dark blue-to-violet colony. Formation of cherry-red color indicates the positive reaction.
09142 ECD ChromoSelect Agar with MUG for microbiology, NutriSelect Plus ECD Agar ChromoSelect with MUG For the detection of Escherichia coli in water and food samples by using a combination of chromogenic and fluorogenic substrate.
70722 E. coli ChromoSelect Agar B for microbiology, NutriSelect Plus E. coli Agar B ChromoSelect E. coli ChromoSelect Agar B is recommended for the detection and enumeration of Escherichia coli in foods without further confirmation on membrane filter or by indole reagent. Most of the Escherichia coli strains can be differentiated from other coliforms by the presence of enzyme glucuronidase which is highly specific for Escherichia coli. Escherichia coli cells absorb X-glucuronide and the intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore gives the blue coloration of the colonies.
44782 E. coli O157:H7 MUG Agar for microbiology   Selective agar for the isolation and differentiation of enterohaemorrhagic (EHEC) E. coli O157:H7-strains from food and clinical material.
E5399 Endo agar for microbiology, NutriSelect Plus    
70137 ENDO Agar (Base) for microbiology, NutriSelect Plus   A selective medium for the differentiation of lactose fermenting and lactose non-fermenting intestinal organisms.
85766 m-Endo Agar LES for microbiology, NutriSelect Plus Membrane Endo Agar LES m-Endo Agar LES can be used for the enumeration of coliforms in water using the Membrane filter technique for coliform enumeration. This method is more reliable and precise than MPN multiple test. It follows the two step membrane filter procedure using Lauryl Sulfate Broth (Cat. No. 17349) as a preliminary enrichment, resulting in higher coliform counts.
96961 m-FC-Agar for microbiology, NutriSelect Plus Membrane Fecal Coliform Agar m-Fecal Coliform Agar m-FC Agar is used for the detection of faecal coliforms by the membrane filtration technique.
43291 m-FC Agar, Vegitone for microbiology, NutriSelect Plus Membrane Fecal Coliform Agar, Vegitone Vegitone m-FC Agar m-Fecal Coliform Agar, Vegitone M-FC Agar is used for the detection and enumeration of faecal coliforms using membrane filter technique at higher temperature. In this modified medium the animal derived peptones are replaced by plant peptones.
48716 Gassner Agar for microbiology, NutriSelect Basic Water Blue Metachrome-yellow Lactose Agar according to Gassner Selective agar for the detection and isolation of pathogenic Enterobacteriaceae in food and other material according to Gassner.
54232 Lactose TTC Agar with Tergitol-7 for microbiology, NutriSelect Plus TTC-Tergitol-7 Agar m-Tergitol-7 Agar with Meat Extract for detection and enumeration of coliform organisms from chlorinated water by membrane filter technique
07348 m-Lauryl sulfate broth for microbiology, NutriSelect Plus Membrane lauryl sulfate broth M-Lauryl sulfate broth is used for enumeration of Escherichia coli and coliforms in water, using membrane filter technique, replacing membrane enriched teepol broth. It is recommended by ISO Committee under the specification ISO 9308-1: 1990
62087 Levine EMB Agar for microbiology, NutriSelect Plus Eosin methylene Blue Lactose Agar according to Levine Levine’s Eosin Methylene Blue Agar Isolation and differentiation of E. coli and Enterobacter, and identification of Candida albicans according to Levine.
62634 LST-MUG Broth for microbiology, NutriSelect Plus   Fluorescent method for the detection of E. coli.
LST-MUG broth is a growth media, which is used for the selective detection and differentiation of Escherichia coli in water samples.
70143 MacConkey Agar No 1 for microbiology, NutriSelect Plus   A differential plating medium recommended for the detection and isolation of coliforms and intestinal pathogens from stool, urine, water and other material.
M8302 MacConkey Agar with Crystal violet, Sodium chloride and 0.15% Bile salts for microbiology, NutriSelect Plus   A differential media recommended for the selection and recovery of Enterobacteriaceae.
51405 MacConkey-Agar (without salt) for microbiology, NutriSelect Plus MacConkey-Agar (without salt and crystal violet) A differential medium which restricts the swarming of most Proteus species. Useful for culturing urine specimens which may contain large number of Proteus species.
70144 MacConkey Broth for microbiology, NutriSelect Plus   A presumptive medium for the detection of the coliform group in water and milk.
16377 MacConkey Broth Purple for microbiology, NutriSelect Plus MacConkey Bromocresol purple Broth MacConkey G Broth MacConkey Broth Purple is a differential medium used for the detection of coliforms in water samples and milk examination by MPN technique. It is a modification of the classic medium, where neutral red is replaced by a less aggressive indicator, according European Pharmacopoeia. It is also recommended by ISO Committee under the specification ISO 9308-2:1990.
63014 MacConkey MUG Agar for microbiology, NutriSelect Plus   For the isolation of Salmonella, Shigella and coliform bacteria, in particular E. coli, from diverse material.
Bile salts and crystal violet extensively inhibit the Gram-positive flora. The presence of lactose and neutral red indicate lactose-positive colonies from which E. coli can be identified by fluorescence in the UV.
88902 MacConkey-Sorbitol Agar for microbiology, NutriSelect Plus   MacConkey-Sorbitol Agar is a growth media, which is used for the selective detection and differentiation of Escherichia coli in water samples.
83339 Mac Conkey-Sorbitol ChromoSelect Agar for microbiology, NutriSelect Plus Mac Conkey-sorbitol Agar ChromoSelect SMAC Agar ChromoSelect Sorbitol MacConkey Agar ChromoSelect Mac Conkey-Sorbitol ChromoSelect Agar is recommended for selective isolation of Escherichia coli 0157:H7 from food and animal feeding stuffs. MacConkey Sorbitol Agar is based on the formulation described by Rappaport and Henigh. The medium contains sorbitol instead of lactose and it is recommended for the detection of enteropathogenic strains of E. coli 0157:H7 which ferments lactose but does not ferment sorbitol and hence produce colorless colonies. Sorbitol fermenting strains of E. coli produce pink-red colonies. The red color is due to production of acid from sorbitol, absorption of neutral red and a subsequent color change of the dye when pH of the medium falls below 6.8. E. coli 0157:H7 has been recognised as a cause of haemorrhagic colitis. B.C. indicator is added to detect the presence of an enzyme β-D-glucuronidase which is specific for E. coli. Stranins of E. coli possessing β-D-glucuronidase appear as blue colored colonies on the medium. Enteropathogenic strains of E. coli 0157 do not possess beta-D-glucuronidase activity and thus produce colorless colonies. E. coli fermenting sorbitol and possessing beta-D-glucuronidase activity produce purple colored colonies. Most of the gram-positive organisms are inhibited by crystal violet and bile salts. Addition of Tellurite-Cefixime Supplement makes the medium selective. Potassium Tellurite selects the serogroups 0157 from other E. coli serogroups and inhibits Aeromonas species and Providencia species. Cefixime inhibits Proteus species.
39734 Membrane Lactose Glucuronide Agar for microbiology, NutriSelect Plus MLGA m-Lauryl Sulfate Chromogen Agar A chromogenic medium for the differentiation and enumeration of Escherichia coli and other coliforms, which simplifies the membrane filtration technique by reducing the number of filtration stages and the need for further confirmation steps. This medium is described in the Environment Agency′s report of UK, ′Methods for Examination of Waters and Associated Material - The Microbiology of Drinking Water (2002) - Part 4 - Methods for the isolation and enumeration of coliform bacteria and Escherichia coli (including E. coli O157:H7).
M1678 MUG EC broth for microbiology, NutriSelect Plus 4-Methylumbelliferyl β-D-Glucuronide Escherichia coli Broth Used for the detection of E. coli by a fluorogenic procedure.
51489 Rapid Coliform ChromoSelect Broth for microbiology, NutriSelect Plus Coliform Rapid ChromoSelect Broth Rapid Coliform ChromoSelect Broth is used for detection and conformation of Escherichia coli and coliforms on the basics of enzyme substrate reaction from water samples, using a combination of chromogenic and fluorogenic substrate.
84369 Salmonella Chromogen Agar for microbiology, NutriSelect Basic   A differential diagnostic agar for the detection of Salmonella in food and clinical material. A modification of the formulation described by Rambach (1990).
01993 Salmonella Chromogen Agar Set for microbiology, NutriSelect Basic   A differential diagnostic agar for the detection of Salmonella in food and clinical material.
05538 Salmonella ChromoSelect Agar, Improved for microbiology, NutriSelect Plus Salmonella Agar ChromoSelect, Improved A selective chromogenic medium used for simultaneous detection of Salmonella and Escherichia coli from food and water samples.
92435 TBX Agar for microbiology, NutriSelect Plus Tryptone Bile X-glucuronide Agar Chromogenic Agar for the detection and enumeration of E. coli in foodstuffs, animal food and water.
90924 m-TEC ChromoSelect Agar for microbiology, NutriSelect Plus   m-TEC ChromoSelect Agar is recommended by the U.S. Environmental Protection Agency (USEPA) for differentiation and enumeration of thermotolerant Escherichia coli from water by membrane filtration technique. m-TEC ChromoSelect Agar has been used for membrane filtration of samples from the rivers, lakes and wetlands for subsequent isolation and culturing of Escherichia coli.
86455 Tergitol-7 Agar for microbiology, NutriSelect Plus   Selective medium for the detection of coliform bacteria and for early detection of E. coli in water analysis. For the detection of fecal contamination in foods according to Mossel(1962).
70188 Violet Red Bile Agar for microbiology, NutriSelect Plus VRB Agar VRBL-Agar A selective medium for the detection and enumeration of coliform organisms, especially the Coli-aerogenes-group in water, milk and other dairy products.
17213 Violet Red Bile Dextrose Agar for microbiology, NutriSelect Plus VRBD Agar Violet Red Bile Glucose Agar without Lactose Violet Red Bile Glucose Agar is used for detection and enumeration of Enterobacteriaceae in food products. It is also recommended by ISO Committee under the specifications 21528-1:2017 and 21528-2:2017.
70189 Violet Red Bile Glucose Agar for microbiology, NutriSelect Plus VRBG-Agar A selective medium for the isolation and enumeration of enterobacteria.
42376 Violet Red Bile Lactose Agar, Vegitone for microbiology, NutriSelect Plus Crystal violet-Neutral red-Bile Agar, Vegiton VRB-Agar, Vegitone Vegitone Violet Red Bile Agar Violet Red Bile Agar Vegitone is a selective media used for the detection and enumeration of coliform organisms from water and food. This medium contains no animal derived peptone and bile salt.
17215 WL Differential Agar for microbiology, NutriSelect Plus WLD Agar Wallerstein Differential Agar WL Differential Agar has been used for the enumeration of Kluyveromyces marxianus culture.
76721 XLT4 Agar (Base) for microbiology, NutriSelect Plus Xylose Lysine Tergitol-4 Agar (Base) for the isolation and identification of pathogenic Enterobacteriaceae, especially Salmonella species