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Materials ### related product Product No. Description Pricing ### [53778-U](https://www.sigmaaldrich.com/US/en/product/supelco/53778u) ### Ascentis® Express OH5, 2.7 μm HPLC Column 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm Expand View Pricing ## CONDITIONS ### column Ascentis Express OH-5, 15 cm x 4.6 mm, I.D., 2.7 μm particles (53778-U) ### mobile phase \[A] 0.005 M ammonium formate, pH 3.15 with concentrated TFA \[B] acetonitrile (30:70, A:B) ### flow rate 0.5 mL/min ### pressure 841 psi (58 bar) ### column temp. 35 °C ### detector ELSD (detector other: Nebulizer 60º C, Evaporator 80º C, Gas flow 1.60 SLM (Standard Liters/ Minute), LED 100%, Smoothing 5.0 S, PMT ( Photomultiplier) Gain 1.0) ### injection 10 μL ### sample 0.5 mg/mL each prepared in 50:50 Water:Acetonitrile ## Description ### Analysis Note This application shows the analysis of sugars by HILIC Chromatography with ELSD detection. The purpose of the work shown here was to develop a HILIC method that could quickly separate mono- and di-saccharides with good sensitivity and reproducibility utilizing the Ascentis Express OH-5 column and an Evaporative Light Scattering Detecctor (ELSD) detector. ### Legal Information Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany [Chromatogram Search](https://www.sigmaaldrich.com/US/en/chromatogram-search) ### Related Product Categories [HPLC Columns](https://www.sigmaaldrich.com/US/en/products/analytical-chemistry/analytical-chromatography/hplc-columns)[GC Columns & Accessories](https://www.sigmaaldrich.com/US/en/products/analytical-chemistry/analytical-chromatography/gc-columns-accessories-and-adsorbents)[TLC Plates & Adsorbents](https://www.sigmaaldrich.com/US/en/products/analytical-chemistry/analytical-chromatography/tlc-plates-and-adsorbants)[Solid Phase Microextraction (SPME)](https://www.sigmaaldrich.com/US/en/products/analytical-chemistry/analytical-sample-preparation/spme-fibers-and-accessories)[Solid Phase Extraction & QuEChERS](https://www.sigmaaldrich.com/US/en/products/analytical-chemistry/analytical-sample-preparation/spe-and-quechers) ### Related Applications [Small Molecule HPLC](https://www.sigmaaldrich.com/US/en/applications/analytical-chemistry/liquid-chromatography/small-molecule-hplc)[Large Molecule HPLC](https://www.sigmaaldrich.com/US/en/applications/analytical-chemistry/liquid-chromatography/large-molecule-hplc)[Gas Chromatography (GC)](https://www.sigmaaldrich.com/US/en/applications/analytical-chemistry/gas-chromatography)[TLC](https://www.sigmaaldrich.com/US/en/applications/analytical-chemistry/thin-layer-chromatography)[Solid Phase Microextraction (SPME)](https://www.sigmaaldrich.com/US/en/applications/analytical-chemistry/sample-preparation/solid-phase-microextraction)[Solid Phase Extraction (SPE)](https://www.sigmaaldrich.com/US/en/applications/analytical-chemistry/sample-preparation/solid-phase-extraction)[QuEChERS](https://www.sigmaaldrich.com/US/en/applications/analytical-chemistry/sample-preparation/quechers) Support[Customer Support](https://www.sigmaaldrich.com/US/en/support/customer-support)[Contact Us](https://www.sigmaaldrich.com/US/en/collections/offices)[FAQ](https://maestro.my.site.com/knowledgeportal/s/)[Safety Data Sheets (SDS)](https://www.sigmaaldrich.com/US/en/documents-search?tab=sds)[Certificates (COA/COO)](https://www.sigmaaldrich.com/US/en/documents-search?tab=coa)[Quality & Regulatory](https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management)[Calculators & Apps](https://www.sigmaaldrich.com/US/en/support/calculators-and-apps)[Webinars](https://www.sigmaaldrich.com/US/en/collections/webinars) Orders[Order Lookup](https://www.sigmaaldrich.com/US/en/order-lookup)[Quick Order](https://www.sigmaaldrich.com/US/en/quick-order)[Custom Products](https://www.sigmaaldrich.com/US/en/services/custom-products)[eCommerce Solutions](https://www.sigmaaldrich.com/US/en/life-science/ecommerce/ecommerce-solutions) Company[About Us](https://www.sigmaaldrich.com/US/en/life-science/about-us)[Responsibility](https://www.sigmaaldrich.com/US/en/life-science/ssbi)[Events](https://www.sigmaaldrich.com/US/en/collections/events)[Press Releases](https://www.sigmaaldrich.com/US/en/collections/press)[Programs](https://www.sigmaaldrich.com/US/en/life-science/partnership-programs)[Careers](https://careers.merckgroup.com/global/en)[Offices](https://www.sigmaaldrich.com/US/en/collections/offices) Social Media [](https://www.linkedin.com/company/milliporesigma) [](https://x.com/MilliporeSigma) [](https://www.facebook.com/MilliporeSigma) [](https://www.instagram.com/milliporesigma/) [](https://www.youtube.com/@milliporesigma2407/featured) MilliporeSigma __Research. 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