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[Home](https://www.sigmaaldrich.com/CA/en)[Applications](https://www.sigmaaldrich.com/CA/en/applications)[Microbiological Testing](https://www.sigmaaldrich.com/CA/en/applications/microbiological-testing)Pathogen & Spoilage Testing
# Pathogen & Spoilage Testing
Pathogen testing is used to identify pathogenic organisms in manufacturing environments, ingredients or finished products that could harm the consumer. Types of pathogens include specific bacteria strains, viruses, fungi, or parasites that can cause illness in its host. Pathogen testing is continuously performed throughout the production process from raw materials to finished product screening to ensure consumer safety and is subject to strict food safety regulatory guidelines.
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## Related Products
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[
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Millipore
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71008BC
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GDS *Salmonella* Tq](https://www.sigmaaldrich.com/CA/en/product/sial/71008bc)
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Millipore
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71007BC
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GDS *E. coli* O157:H7 Tq](https://www.sigmaaldrich.com/CA/en/product/sial/71007bc)
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[
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Millipore
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1.32359
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MC-Media Pad® RAC](https://www.sigmaaldrich.com/CA/en/product/mm/132359)
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Millipore
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1.32357
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MC-Media Pad® EC](https://www.sigmaaldrich.com/CA/en/product/mm/132357)
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[
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Millipore
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66004BC
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SimPlate® Yeast & Mold](https://www.sigmaaldrich.com/CA/en/product/sial/66004bc)
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[
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Millipore
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60037BC
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VIP® Gold *Listeria*](https://www.sigmaaldrich.com/CA/en/product/sial/60037bc)
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Millipore
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61001BC
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TRANSIA® AG *Salmonella*](https://www.sigmaaldrich.com/CA/en/product/sial/61001bc)
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Millipore
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5.42753
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DiluCult™](https://www.sigmaaldrich.com/CA/en/product/mm/542753)
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Millipore
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5.42765
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Enrichment Sample Homogenizer (ESH)](https://www.sigmaaldrich.com/CA/en/product/mm/542765)
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Millipore
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1.46403
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Buffered Peptone Water](https://www.sigmaaldrich.com/CA/en/product/mm/146403)
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Millipore
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1.46431
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Tryptic Soy Agar - Ready-to-use Settle Plates](https://www.sigmaaldrich.com/CA/en/product/mm/146431)
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Millipore
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1.07228
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Buffered Peptone Water](https://www.sigmaaldrich.com/CA/en/product/mm/107228)
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Millipore
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1.05463
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Plate Count agar](https://www.sigmaaldrich.com/CA/en/product/mm/105463)
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Millipore
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1.04143
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Singlepath® *Campylobacter*](https://www.sigmaaldrich.com/CA/en/product/mm/104143)
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Millipore
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1.04148
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Singlepath® *Listeria monocytogenes*](https://www.sigmaaldrich.com/CA/en/product/mm/104148)
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Millipore
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1.01865
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Readybag® Half Fraser Broth with Supplements](https://www.sigmaaldrich.com/CA/en/product/mm/101865)
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## Featured Categories
[](https://www.sigmaaldrich.com/CA/en/products/industrial-microbiology/microbial-culture-media)
[Microbial Culture Media](https://www.sigmaaldrich.com/CA/en/products/industrial-microbiology/microbial-culture-media)
Discover high-quality microbial culture media. Choose from dehydrated or ready-to-use options, meeting industry standards and regulatory requirements.
[Shop Products](https://www.sigmaaldrich.com/CA/en/products/industrial-microbiology/microbial-culture-media)
[](https://www.sigmaaldrich.com/CA/en/products/industrial-microbiology/pathogen-screening-kits-instuments-and-disposables)
[Pathogen Screening Kits, Instruments & Disposables](https://www.sigmaaldrich.com/CA/en/products/industrial-microbiology/pathogen-screening-kits-instuments-and-disposables)
Pathogen testing is a key component and critical success factor in effective food safety and process control programs. Explore our wide range of pathogen screening products that suit your needs.
[Shop Products](https://www.sigmaaldrich.com/CA/en/products/industrial-microbiology/pathogen-screening-kits-instuments-and-disposables)
[](https://www.sigmaaldrich.com/CA/en/products/industrial-microbiology/microbial-culture-media-raw-materials)
[Microbial Culture Media Raw Materials](https://www.sigmaaldrich.com/CA/en/products/industrial-microbiology/microbial-culture-media-raw-materials)
Explore our wide range of culture media raw materials and supplements for microbiological purposes. All our raw materials and culture media supplements are safe to handle and ensure optimal performance.
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[](https://www.sigmaaldrich.com/CA/en/products/industrial-microbiology/quality-indicator-testing-devices)
[Quality Indicator Testing Devices](https://www.sigmaaldrich.com/CA/en/products/industrial-microbiology/quality-indicator-testing-devices)
Our collection of testing systems includes alternative & rapid culture media for fast & reliable indicator organisms testing results in a time-efficient method.
[Shop Products](https://www.sigmaaldrich.com/CA/en/products/industrial-microbiology/quality-indicator-testing-devices)
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Overview
Related Articles & Protocols
Support
## [](https://www.sigmaaldrich.com)BIOSAFETY TESTING SERVICES
[Biosafety testing services](https://www.sigmaaldrich.com/CA/en/campaigns/bioreliance-biosafety-testing-and-product-characterization) to investigate mAbs and cell and gene therapy and vaccines materials to ensure that they are free of adventitious agents or unexpected results that can cause catastrophic failures in downstream processing, including: Microbiology and Molecular testing.
## [](https://www.sigmaaldrich.com)Pathogen Detection Methods
- Conventional cultivation-based enrichment and plating methods and rapid plating solution
- Ready-to-use media methods
- ELISA and immunoassay technology
- PCR and molecular detection
- Lateral flow tests
## [](https://www.sigmaaldrich.com)Indicator Organisms
While pathogen testing is looking for specific pathogens, indicator organism testing is looking to verify overall sanitation and safety. Microbial indicators are microorganisms that are not necessarily pathogenic but indicate the potential presence of pathogens. Detecting indicator organisms means that the product has been exposed to conditions that could lead to an increased risk of pathogenic contamination. Pathogens can be difficult to detect. Therefore, indicator organism tests are often used as additional methods to assess the hygienic conditions of a product or manufacturing environment.
## [](https://www.sigmaaldrich.com)Microbial Spoilage Testing
Microbial spoilage testing is the process of determining the presence of spoilage microorganisms that cause product deterioration, effecting the quality and safety. These include Gram-positive and Gram-negative bacteria, as well as yeasts and molds. Spoilage microorganisms are not pathogenic, but can change the physical properties, such as texture, smell, taste, and color, or chemical properties of a product and making it unsuitable for consumption. Spoilage can occur at any stage along the food chain and needs to be part of the microbiological quality control. The testing requirements vary from industry to industry, depending on different spoilage parameters.
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## Related Articles
- [Differentiation and Detection of Escherichia coli, Coliforms and Enterobacteriaceae](https://www.sigmaaldrich.com/CA/en/technical-documents/technical-article/microbiological-testing/pathogen-and-spoilage-testing/selective-growth-media)
An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.
- [Microbiology Quality Control in Food and Beverage](https://www.sigmaaldrich.com/CA/en/technical-documents/technical-article/microbiological-testing/pathogen-and-spoilage-testing/microbio-qc-food-and-beverage)
Food contaminated by microorganisms (bacteria and yeasts), viruses, and protozoa can cause severe disease.
- [HybriScan® Rapid Microbial Test System](https://www.sigmaaldrich.com/CA/en/technical-documents/technical-article/microbiological-testing/pathogen-and-spoilage-testing/hybriscan-rapid-test)
Fast molecular screening of beverage, water, wastewater and food for microorganisms
- [*Salmonella* Contamination and Rapid Detection Methods](https://www.sigmaaldrich.com/CA/en/technical-documents/technical-article/microbiological-testing/pathogen-and-spoilage-testing/salmonella-contamination-and-testing)
Salmonella pass through the entire food chain from animal feed, primary production, and into households or food-service establishments.
- [Detection of Listeria monocytogenes in Food and Environmental Samples](https://www.sigmaaldrich.com/CA/en/technical-documents/technical-article/microbiological-testing/pathogen-and-spoilage-testing/listeria-monocytogenes)
Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS
- [See All (42)](https://www.sigmaaldrich.com/CA/en/search/facet-search?focus=sitecontent&term=facet-search)
## Related Protocols
- [Study: Comparison of MC-Media Pad® with Different Convenience Solution Products](https://www.sigmaaldrich.com/CA/en/technical-documents/protocol/microbiological-testing/microbial-culture-media-preparation/mc-media-pad-product-comparison)
Comparison of MC-Media Pad® with different convenience solution products for indicator organism testing
- [Yeast Growth Methods](https://www.sigmaaldrich.com/CA/en/technical-documents/protocol/microbiological-testing/pathogen-and-spoilage-testing/yeast-growth-protocols)
Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.
- [Revised EN ISO 21528:2017 for Detection and Enumeration of Enterobacteriaceae in the Food Chain](https://www.sigmaaldrich.com/CA/en/technical-documents/protocol/food-and-beverage-testing-and-manufacturing/regulatory-compliance-for-food-and-beverage/iso-21528-enterobacteriaceae-detection-and-enumeration)
The International Organization for Standardization (ISO) published the revised EN ISO 21528-1 and EN ISO 21528-2 standards in 2017.
- [*Salmonella* Detection in the Food Chain (EN ISO 6579-1)](https://www.sigmaaldrich.com/CA/en/technical-documents/protocol/food-and-beverage-testing-and-manufacturing/regulatory-compliance-for-food-and-beverage/iso-6579-1-salmonella-detection)
EN ISO 6579-1 standard specifies detection of Salmonella spp. in human consumption, animal feed, and environmental samples.
- [Validation of Shorter Protocol for Detection of *Salmonella enterica* subsp. *enterica* in Peanut Butter Samples Followed by a rRNA Detection System](https://www.sigmaaldrich.com/CA/en/technical-documents/protocol/microbiological-testing/pathogen-and-spoilage-testing/protocol-for-detection-of-salmonella-enterica)
Food-borne pathogens Salmonella is commonly evaluated in manufacturing of peanut butter and other food products.
- [See All (11)](https://www.sigmaaldrich.com/CA/en/search/facet-search?focus=sitecontent&term=facet-search)
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__Featured Articles__
[Differentiation and Detection of Escherichia coli, Coliforms and Enterobacteriaceae](https://www.sigmaaldrich.com/CA/en/technical-documents/technical-article/microbiological-testing/pathogen-and-spoilage-testing/selective-growth-media)
An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.
[Molecular Detection for Rapid Food Pathogen Testing](https://www.sigmaaldrich.com/CA/en/technical-documents/technical-article/microbiological-testing/pathogen-and-spoilage-testing/assurance-gds-genetic-detection)
Assurance® GDS is an integrated solution for food pathogen detection. It offers faster pathogen testing results with high accuracy to meet the demanding needs of food, environmental and cannabis microbiology testing challenges.
[*Salmonella* Contamination and Rapid Detection Methods](https://www.sigmaaldrich.com/CA/en/technical-documents/technical-article/microbiological-testing/pathogen-and-spoilage-testing/salmonella-contamination-and-testing)
Salmonella pass through the entire food chain from animal feed, primary production, and into households or food-service establishments.
[Anaerobic Culture with Anaerocult®](https://www.sigmaaldrich.com/CA/en/technical-documents/technical-article/microbiological-testing/pathogen-and-spoilage-testing/anaerobic-incubation-system)
Anaerocult® system supports reliable anaerobe cultivation for microbiological research needs.
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