¿Você se preocupa em como prepara sua amostra biológica?

Em estatística, resultados falsos positivos e falsos negativos ocorrem quando um resultado positivo corrobora a hipótese nula e o resultado negativo corrobora para não rejeitar essa hipótese.

Em amostras biológicas, como o plasma e sangue humano total, por serem muito complexas, algum tipo de preparação de amostra deve ser feito para minimizar que erros como esses ocorram acima de uma frequência pré-definida.


Área Título Autor Ano Revista
BIOTECNOLOGIA   An open-source solution for advanced imaging flow cytometry data analysis using machine learning Emily Staples,1 Richard James Michael Ingram,1 John Christopher Atherton, E Karen Robinson 2013 JOURNAL ELSEVIER
BIOTECNOLOGIA   Flow cytometry what you see matters: Enhanced clinical detection using image-based flow cytometry Juliane Midori Ikebara, Silvia Honda Takada, Débora Sterzeck Cardoso, Natália Myuki Moralles Dias, Beatriz Crossiol Vicente de Campos, Talitha Amanda Sanches Bretherick, Guilherme Shigueto Vilar Higa, Mariana Sacrini Ayres Ferraz, e Alexandre Hiroaki Kihara 2013 JOURNAL PLOS
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BIOTECNOLOGIA   Characterization of neutrophils and macrophages from ex vivo-cultured murine bone marrow for morphologic maturation and functional responses by imaging flow cytometry Venina M. Dominical, Leigh Samsel, James S. Nichols, Fernando F. Costa, J. Phillip McCoy, Nicola Conran, Gregory J. Kato 2014 JOURNAL OF THE EUROPEAN HEMATOLOGY ASSOCIATION
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BIOTECNOLOGIA   Qualitative and quantitative analysis of PMN/T-cell interactions by InFlow and super-resolution microscopy Rodrigues MA, Beaton-Green LA, Wilkins RC. 2016 MEDLINE
BIOTECNOLOGIA   Retinal transplantation of photoreceptors results in donor-host cytoplasmic exchange Sheyn U, Rosenwasser S, Ben-Dor S, Porat Z, Vardi A 2016 NATURE
BIOTECNOLOGIA   Imaging flow cytometry in the assessment of leukocyte-platelet aggregates Dixita I. Viswanath, Emily M. Mace, Hsiang-Ting Hsu, Jordan S. Orange 2017 JOURNAL ELSEVIER
BIOTECNOLOGIA   Imaging flow cytometry for the characterization of extracellular vesicles Verma AH, Bueter CL, Rothenberg ME, Deepe GS 2017 NATURE - MUCOSAL IMMUNOLOGY
BIOTECNOLOGIA   E2F-2 Promotes Nuclear Condensation and Enucleation of Terminally Differentiated Erythroblasts Wang W, Burg N, Vootukuri S, Coller BS. 2016 JOURNAL ELSEVIER
BIOTECNOLOGIA   Immobilization and detection of platelet-derived extracellular vesicles on functionalized silicon substrate: cytometric and spectrometric approach Cezary Treda , Marta Popeda, Magdalena Ksiazkiewicz, Dawid P. Grzela, Maciej P. Walczak, Mateusz Banaszczyk, Joanna Peciak, Ewelina Stoczynska-Fidelus, Piotr Rieske 2016 JOURNAL PLOS
BIOTECNOLOGIA   Immobilization and detection of platelet-derived extracellular vesicles on functionalized silicon substrate: cytometric and spectrometric approach Rahul Roychoudhuri, David Clever, Peng Li, Yoshiyuki Wakabayashi, Kylie M Quinn, Christopher A Klebanoff, Yun Ji, Madhusudhanan Sukumar, Robert L Eil, Zhiya Yu, Rosanne Spolski, Douglas C Palmer, Jenny H Pan, Shashank J Patel, Derek C Macallan, Giulia Fabozzi, Han-Yu Shih, Yuka Kanno, Akihiko Muto, Jun Zhu, Luca Gattinoni, John J O'Shea, Klaus Okkenhaug, Kazuhiko Igarashi, Warren J Leonard  2016 NATURE - IMMUNOLOGY
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BIOTECNOLOGIA   Interleukin-33-induced expression of PIBF1 by decidual B cells protects against preterm labor Romina F. Vázquez, Sabina M. Maté, Laura S. Bakás, Carlos Muñoz-Garay, Vanesa S. Herlax 2016 JOURNAL ELSEVIER
BIOTECNOLOGIA   Renal Denervation Reduces Monocyte Activation and Monocyte-Platelet Aggregate Formation: An Anti-Inflammatory Effect Relevant for Cardiovascular Risk Dashkova V, Malashenkov D, Poulton N, Vorobjev I, Barteneva NS 2017 JOURNAL ELSEVIER
BIOTECNOLOGIA   Silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesis Haley R. Pugsley 2017 JOURNAL ELSEVIER
BIOTECNOLOGIA   Directed differentiation of human iPSC into insulin producing cells is improved by induced expression of PDX1 and NKX6.1 factors in IPC progenitors Maguire O, Chen GL, Hahn TE, Brix L, McCarthy PL, Wallace PK, Minderman H. 2017 JOURNAL ELSEVIER
BIOTECNOLOGIA   HIV Infects Bronchial Epithelium and Suppresses Components of the Mucociliary Clearance Apparatus Vorobjev IA, Barteneva NS 2017 JOURNAL ELSEVIER
BIOTECNOLOGIA   Plasma from pre-pubertal obese children impairs insulin stimulated Nitric Oxide (NO) bioavailability in endothelial cells: Role of ER stress Dominical V, Samsel L, McCoy JP Jr   JOURNAL ELSEVIER
BIOTECNOLOGIA   The random co-polymer glatiramer acetate rapidly kills primary human leukocytes through sialic-acid-dependent cell membrane damage Széliová D, Krahulec J, Šafránek M, Lišková V, Turňa J 2016 JOURNAL ELSEVIER
BIOTECNOLOGIA   Connected component masking accurately identifies the ratio of phagocytosed and cell-bound particles in individual cells by imaging flow cytometry Luk HY, McFarlin BK, Vingren JL 2017 JOURNAL ELSEVIER
BIOTECNOLOGIA   Docosahexaenoic acid loaded lipid nanoparticles with bactericidal activity against Helicobacter pylori Wang D, Li XR, Dong DJ, Huang H, Wang JX, Zhao XF 2016 JBC
BIOTECNOLOGIA   hucMSC Exosome-Derived GPX1 Is Required for the Recovery of Hepatic Oxidant Injury Stojanovic T, Orlova M, Sialana FJ, Höger H, Stuchlik S, Milenkovic I, Aradska J, Lubec G. 2017 JOURNAL AMINO ACIDS
BIOTECNOLOGIA   The anti-inflammatory Annexin A1 induces the clearance and degradation of the amyloid-beta peptide Vanessa Lagal, Márcia Dinis, Dominique Cannella, Daniel Bargieri, Virginie Gonzalez, Nicole Andenmatten, Markus Meissner e Isabelle Tardieux 2015 INFECTION AND IMMUNITY
BIOTECNOLOGIA   Cytotoxicity of withasteroids: withametelin induces cell cycle arrest at G2/M phase and mitochondria-mediated apoptosis in non-small cell lung cancer A549 cells Welinder C, Jirström K, Lehn S, Nodin B, Marko-Varga G, Blixt O, Danielsson L, Jansson B 2016 HELIYON
BIOTECNOLOGIA   Inherent low Erk and p38 activity reduce Fas Ligand expression and degranulation in T helper 17 cells leading to activation induced cell death resistance Tay MZ, Liu P, Williams LD, McRaven MD, Sawant S, Gurley TC, Xu TT, Dennison SM, Liao HX, Chenine AL, Alam SM, Moody MA, Hope TJ, Haynes BF, Tomaras GD. 2016 JOURNAL PLOS
BIOTECNOLOGIA   Sialic acid-modified antigens impose tolerance via inhibition of T-cell proliferation and de novo induction of regulatory T cells Hennig H, Rees P, Blasi T, Kamentsky L, Hung J, Dao D, Carpenter AE, Filby A. 2017 JOURNAL ELSEVIER
BIOTECNOLOGIA   Sialic acid-modified antigens impose tolerance via inhibition of T-cell proliferation and de novo induction of regulatory T cells Haridas V, Ranjbar S, Vorobjev IA, Goldfeld AE, Barteneva NS. 2017 JOURNAL ELSEVIER
BIOTECNOLOGIA   Highly Effective Dual-Function Near-Infrared (NIR) Photosensitizer for Fluorescence Imaging and Photodynamic Therapy (PDT) of Cancer Pelletier MG, Szymczak K, Barbeau AM, Prata GN, O'Fallon KS, Gaines P. 2017 JOURNAL ELSEVIER
BIOTECNOLOGIA   Imagestream detection and characterisation of circulating tumour cells - A liquid biopsy for hepatocellular carcinoma Sarenac T, Trapecar M, Gradisnik L, Rupnik MS, Pahor D. 2016 NATURE
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BIOTECNOLOGIA   Transcriptional Activation of Pericentromeric Satellite Repeats and Disruption of Centromeric Clustering upon Proteasome Inhibition Swartz KL, Wood SN, Murthy T, Ramirez O, Qin G, Pillai MM4, Rao S, Minella AC. 2016 JOURNAL ASM 
BIOTECNOLOGIA   Applications of Imaging Flow Cytometry in the Diagnostic Assessment of Acute Leukaemia Gajos K, Kamińska A, Awsiuk K, Bajor A, Gruszczyński K, Pawlak A, Żądło A, Kowalik A, Budkowski A, Stępień E. 2017 ANALYTICAL AND BIOANALYTICAL CHEMISTRY
BIOTECNOLOGIA   Identification and classification of the malaria parasite blood developmental stages, using imaging flow cytometry Xue D, Kaufman GN, Dembele M, Beland M, Massoud AH, Mindt BC, Fiter R, Fixman ED, Martin JG, Friedel RH, Divangahi M, Fritz JH, Mazer BD. 2017 THE JOURNAL OF IMMUNOLOGY
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BIOTECNOLOGIA   Determination of Levetiracetam in Human Plasma by Dispersive Liquid-Liquid Microextraction Followed by Gas Chromatography-Mass Spectrometry Yang T, Yao Q, Cao F, Liu Q, Liu B, Wang XH. 2016 JOURNAL OF NANOMEDICINE
BIOTECNOLOGIA   Powdered Activated Carbon: An Alternative Approach to Genomic DNA Purification Chinnapaiyan S, Parira T, Dutta R, Agudelo M, Morris A, Nair M, Unwalla HJ. 2017 JOURNAL PLOS
BIOTECNOLOGIA   Green Synthesized Zinc Oxide (ZnO) Nanoparticles Induce Oxidative Stress and DNA Damage in Lathyrus sativus L Di Pietro N, Marcovecchio ML, Di Silvestre S, de Giorgis T, Cordone VG, Lanuti P, Chiarelli F, Bologna G, Mohn A, Pandolfi A. 2017 JOURNAL ELSEVIER
BIOTECNOLOGIA   Development of an immunochromatographic strip test for the rapid detection of zearalenone in wheat Fei C, Lillico DME, Hall B, Rieger AM, Stafford JL. 2017 JOURNAL OF ISAC
BIOTECNOLOGIA   Photolysis of sulfamethoxypyridazine in various aqueous media: aerobic biodegradation and identification of photoproducts by LC-UV-MS/MS Ries M, Loiola R, Shah UN, Gentleman SM, Solito E, Sastre M. 2016 JOURNAL OF NEUROINFLAMMATION
BIOTECNOLOGIA   Occurrence of fungi and cytotoxicity of the species: Aspergillus ochraceus, Aspergillus niger and Aspergillus flavus isolated from the air of hospital wards Peroumal D, Abimannan T, Tagirasa R, Parida JR, Singh SK, Padhan P, Devadas S. 2016 ONCOTARGET
BIOTECNOLOGIA   Detection of pathogenic bioaerosols and occupational risk in a Philippine landfill site Perdicchio M1, Ilarregui JM, Verstege MI, Cornelissen LA, Schetters ST, Engels S, Ambrosini M, Kalay H, Veninga H, den Haan JM, van Berkel LA, Samsom JN, Crocker PR, Sparwasser T, Berod L, Garcia-Vallejo JJ, van Kooyk Y, Unger WW. 2016 PNAS
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BIOTECNOLOGIA   Conversion L-tryptophan to melatonin in the gastrointestinal tract: the new high performance liquid chromatography method enabling simultaneous determination of six metabolites of L-tryptophan by native fluorescence and UV-VIS detection de Valle E, Grigoriadis G, O'Reilly LA, Willis SN, Maxwell MJ, Corcoran LM, Tsantikos E, Cornish JK, Fairfax KA, Vasanthakumar A, Febbraio MA, Hibbs ML, Pellegrini M, Banerjee A, Hodgkin PD, Kallies A, Mackay F, Strasser A, Gerondakis S, Gugasyan R. 2016 THE JOURNAL OF EXPERIMENTAL MEDICINE
BIOTECNOLOGIA   HPLC method development, validation, and impurity characterization of a potent antitumor indenoisoquinoline, LMP776 (NSC 725776) de Assis LV, Moraes MN, da Silveira Cruz-Machado S, Castrucci AM. 2016 JOURNAL ELSEVIER
BIOTECNOLOGIA   NFkappaB1 is essential to prevent the development of multiorgan autoimmunity by limiting IL-6 production in follicular B cells Cummings RJ, Barbet G, Bongers G, Hartmann BM, Gettler K, Muniz L, Furtado GC, Cho J, Lira SA, Blander JM. 2016 NATURE
BIOTECNOLOGIA   The effect of white light on normal and malignant murine melanocytes: A link between opsins, clock genes, and melanogenesis Cisáriková A, Barbieriková Z, Janovec L, Imrich J, Hunáková L, Bačová Z, Paulíková H. 2016 JOURNAL ELSEVIER
BIOTECNOLOGIA   Different tissue phagocytes sample apoptotic cells to direct distinct homeostasis programs Church AK, VanDerMeid KR, Baig NA, Baran AM, Witzig TE, Nowakowski GS, Zent CS. 2015 THE JOURNAL OF IMMUNOLOGY
BIOTECNOLOGIA   Acridin-3,6-dialkyldithiourea hydrochlorides as new photosensitizers for photodynamic therapy of mouse leukemia cells Chintalapudi SR, Djenderedjian L, Stiemke AB, Steinle JJ, Jablonski MM, Morales-Tirado VM. 2016 JOURNAL FRONTIERS
BIOTECNOLOGIA   Anti-CD20 monoclonal antibody-dependent phagocytosis of chronic lymphocytic leukaemia cells by autologous macrophages Cardullo N, Pulvirenti L, Spatafora C, Musso N1, Barresi V, Condorelli DF, Tringali C. 2016 JOURNAL OF NATURAL PRODUCTS
BIOTECNOLOGIA   Isolation and Molecular Profiling of Primary Mouse Retinal Ganglion Cells: Comparison of Phenotypes from Healthy and Glaucomatous Retinas Björnsdottir H, Welin A, Dahlgren C, Karlsson A, Bylund J. 2016 JOURNAL ELSEVIER
BIOTECNOLOGIA   Shape-Dependent Activation of Cytokine Secretion by Polymer Capsules in Human Monocyte-Derived Macrophages Bengsch B, Johnson AL, Kurachi M, Odorizzi PM, Pauken KE, Attanasio J, Stelekati E, McLane LM, Paley MA, Delgoffe GM, Wherry EJ. 2016 JOURNAL IMMUNITY
BIOTECNOLOGIA   Dihydrobenzofuran Neolignanamides: Laccase-Mediated Biomimetic Synthesis and Antiproliferative Activity Ates LS, van der Woude AD, Bestebroer J, van Stempvoort G, Musters RJ, Garcia-Vallejo JJ, Picavet DI, Weerd Rv, Maletta M, Kuijl CP, van der Wel NN, Bitter W. 2016 JOURNAL PLOS
BIOTECNOLOGIA   Quantification of heterotypic granule fusion in human neutrophils by imaging flow cytometry Armbruster NS, Richardson JR, Schreiner J, Klenk J, Günter M, Kretschmer D, Pöschel S, Schenke-Layland K, Kalbacher H, Clark K, Autenrieth SE. 2016 THE JOURNAL OF IMMUNOLOGY