Certificate Of Analysis
Product Name Horse Serum,
Donor Herd, USA origin, Heat inactivated, sterile-filtered, suitable for cell culture
Product Number H1138
Product Brand SIGMA
Cell Line Record cell line(s) SP2/0 and MDBK cell lines used.
Sterility No microbial growth detected No microbial growth detected
Mycoplasma None detected (Broth Culture) None detected
Osmolality 260 - 340 mOsm/kg H2O 285 mOsm/kg H2O
Location of Reserve Sample Present Present
Total Protein 6.0 - 7.5 g% 6.5 g%
AVA Bovine Virus Diarrhea - None detected None detected
Chemical Profile Cholesterol - Report result (mg/dL) 74 mg/dL
AVA Cytopathic Effect - None detected None detected
AVA (9CFR 113.53) Equine Herpervirus - None detected None detected
  Equine Infectious Anemia Virus - None,detected None detected
  Equine Viral Arteritis - None detected None detected
Chemical Profile Iron - Report result (mcg/dL) 270 mcg/dL
  Glucose - Report result (mg/dL) 58 mg/dL
AVA Hemadsorption - None detected None detected
Chemical Profile Sodium - Report result (mEq/L) 139 mEq/L
AVA (9CFR 113.53) Rabies - None detected None detected
  Reovirus - None detected None detected
Chemical Profile Triglyceride - Report result (mg/dL) 22 mg/dL
Cloning Efficiency Pass Pass
Electrophoretic Profile Albumin (g/dL) - Record 1.80 g/dL
  Alpha 1 Globulin (g/dL) - Record 0.68 g/dL
  Alpha 2 Globulin (g/dL) - Record 1.66 g/dL
  Beta Globulin (g/dL) - Record 0.98 g/dL
  Gamma Globulin (g/dL) - Record 1.35 g/dL
Mycoplasma None detected (DNA Flourochrome Stain) None detected
pH 7.0 - 8.2 8.0
Endotoxin <= 10 EU/mL <0.5 EU/mL
Hemoglobin Test <= 20 mg% 1 mg%
Serum Performance Testing Pass Pass
Country of Origin Record country of origin United States
Appearance Clear straw to amber liquid Clear straw liquid

C of A comments Country of final product processing: United States
Origin The materials used in this product are of United States origin. The
  source herd was in good health at the time of collection.
Storage Store at -20 C
  For R&D use only. Not for drug, household, or other uses.
Approval Date and Time 11/01/2012 11:25:39
Manufact Date 8/29/2012
Expiratn Date 8/31/2016